- Main
Transcriptional network orchestrating regional patterning of cortical progenitors
- Ypsilanti, Athéna R;
- Pattabiraman, Kartik;
- Catta-Preta, Rinaldo;
- Golonzhka, Olga;
- Lindtner, Susan;
- Tang, Ke;
- Jones, Ian R;
- Abnousi, Armen;
- Juric, Ivan;
- Hu, Ming;
- Shen, Yin;
- Dickel, Diane E;
- Visel, Axel;
- Pennachio, Len A;
- Hawrylycz, Michael;
- Thompson, Carol L;
- Zeng, Hongkui;
- Barozzi, Iros;
- Nord, Alex S;
- Rubenstein, John L
- et al.
Published Web Location
https://doi.org/10.1073/pnas.2024795118Abstract
We uncovered a transcription factor (TF) network that regulates cortical regional patterning in radial glial stem cells. Screening the expression of hundreds of TFs in the developing mouse cortex identified 38 TFs that are expressed in gradients in the ventricular zone (VZ). We tested whether their cortical expression was altered in mutant mice with known patterning defects (Emx2, Nr2f1, and Pax6), which enabled us to define a cortical regionalization TF network (CRTFN). To identify genomic programming underlying this network, we performed TF ChIP-seq and chromatin-looping conformation to identify enhancer-gene interactions. To map enhancers involved in regional patterning of cortical progenitors, we performed assays for epigenomic marks and DNA accessibility in VZ cells purified from wild-type and patterning mutant mice. This integrated approach has identified a CRTFN and VZ enhancers involved in cortical regional patterning in the mouse.
Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.
Main Content
Enter the password to open this PDF file:
-
-
-
-
-
-
-
-
-
-
-
-
-
-