UC San Diego

In this study we show firstly the differences in physiologic response and tissue outcome on two distinct ischemic models, comparing hemodynamic, inflammatory, and histological parameters. The open window chamber model was utilized in Golden Syrian hamsters and tourniquet or compressive ischemic insult was applied to tissue for one hour, followed by reperfusion. Vasodilation, flow, leukocyte recruitment, wall shear rate, capillary perfusion and ultimate tissue outcome were assessed closely over the course of 24 hours post-ischemia. Despite the subtle differences in the hemodynamic response for both models, tissue cell death outcome for the tourniquet model was found to be significantly more pronounced. Secondly, a novel paramagnetic S-nitrosothiol nanoparticle was used with the compressive model in order to validate the oxidative shielding properties of this proposed nanoparticle intervention. The treatment with this NO- releasing Np was shown to increase blood flow and reduce overall inflammatory response, thus demonstrating promise as a therapeutical avenue for ischemia and other inflammatory conditions