UC Irvine

Summary: Quantitative determinations of a number of naturally occurring D‐hexoses and their oligosaccharides can be accomplished by combinations of enzymatic methods without prior separation of the sugars. These spectrophotometric methods employ readily‐available enzymes and commercial reagents. The procedures are particularly useful for the quantitative analysis of fructofuranosides, such as raffinose or stachyose, in the presence of sucrose, glucose and/or fructose; maltose or lactose mixed with galactose and/or glucose; melibiose in mixtures with raffinose, and other combinations.