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Development and characterization of thirteen microsatellite markers for the Fiscal Flycatcher (Sigelus silens) for use in phylogeographic and landscape genetics research

Abstract

The Fiscal Flycatcher, Sigelus silens, is the only representative of a monotypic genus, endemic to Southern Africa, and may represent two cryptic species. Here we describe the development of thirteen microsatellite markers, and characterize polymorphism for each one. We found that all but one of our 13 loci were highly variable, each having five or more alleles. This suggests that these markers will have high variability across the species range and will be of utility in understanding the extent of gene flow among populations.

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