The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 25 years. In September 2015, ESnet and the Office of Biological and Environmental Research (BER), of the DOE Office of Science, organized a review to characterize the networking requirements of the programs funded by the BER program office. BER advances world-class biological and environmental research programs and scientific user facilities to support DOE’s energy, environment, and basic research missions. This report of the review contains a Findings section and documents Case Studies discussed during the Requirements Review.