Here we report on late Pleistocene fossil vertebrates from new and previously described localities in Santa Clara County, California. The three new localities and specimens include: a partial mammoth pelvis from UCMP V91128 (Lawrence Expressway E); a juvenile cranial specimen of Mammuthus columbi from UCMP V99597 (SCVWD “Lupe” Mammoth), now on display at the San Jose Children’s Discovery Center; and a relatively diverse assemblage of medium- to large-sized mammals, including a nearly complete forelimb of Paramylodon harlani from UCMP V99891 (Babcock’s Bones). We also reassess specimens and assemblages from the following eight previously known localities: Molecular Medicine Building (UCMP V90003); Alma Street Underpass at Page Mill Road (USGS M1203); Mountain View Dump (USGS M1227); Sunnyvale Sewer (USGS M1218); Calabaza, Sunnyvale Sewer (USGS M1218A); Matadero Creek (USGS M1001); Matadero Creek, Veteran’s Hospital (USGS M1202); and Milpitas (UCMP V4916). All localities discussed, except Matadero Creek and Veterans Hospital, are mapped in areas with surficial Holocene deposits, and therefore, these Pleistocene-aged assemblages suggest that Pleistocene deposits are closer to the surface in Santa Clara County than may have been previously thought. Material described here highlights the needed resources for mitigation management in Santa Clara County.