We report simulation experiments estimating the uncertainties in California regional fossil fuel 36 and biosphere CO2 exchanges that might be obtained using an atmospheric inverse modeling 37 system driven by the combination of ground-based observations of radiocarbon and total CO2, 38 together with column-mean CO2 observations from NASA’s Orbiting Carbon Observatory 39 (OCO-2). The work includes an initial examination of statistical uncertainties in prior models for 40 CO2 exchange, in radiocarbon-based fossil fuel CO2 measurements, in OCO-2 measurements, 41 and in a regional atmospheric transport modeling system. Using these nominal assumptions for 42 measurement and model uncertainties, we find that flask measurements of radiocarbon and total 43 CO2 at 10 towers can be used to distinguish between different fossil fuel emissions data products 44 for major urban regions of California. We then show that the combination of flask and OCO-2 45 observations yield posterior uncertainties in monthly-mean fossil fuel emissions of ~ 5-10%, 46 levels likely useful for policy relevant evaluation of bottom-up fossil fuel emission estimates. 47 Similarly, we find that inversions yield uncertainties in monthly biosphere CO2 exchange of ~ 48 6%-12%, depending on season, providing useful information on net carbon uptake in 49 California’s forests and agricultural lands. Finally, initial sensitivity analysis suggests that 50 obtaining the above results requires control of systematic biases below approximately 0.5 ppm, 51 placing requirements on accuracy of the atmospheric measurements, background subtraction, and 52 atmospheric transport modeling.