Is there a cognitive bias against absolute synonymy? The current work explored this question via a miniature mini-artificial language experiment featuring iterated learning, which can amplify weak cognitive biases, as languages are sifted through multiple adult learners. Participants were taught two novel synonymous verbs in positive or negative sentences. Afterwards, they had to generalize the words to new positive or negative sentences. These sentences then served as the input sentences for the next participant in the diffusion chain and so on. Despite inconsistent input with regard to positive or negative meaning, participants differentiated the verbs. More than half of participants strongly differentiated them, specializing at least one term. However, transmission did not increase differentiation overall, suggesting that a bias against synonymy may encourage a minimally distinctive difference, not necessarily a systematic one, between synonyms.