The present study demonstrated the in vitro embryotoxicity assessment of gold nanoparticles (AuNPs) and copper nanoparticles (CuNPs) prepared from the leaves extract of Angelica keiskei (Miq.) Koidz. and addressed their mode of antibacterial mechanisms. Both AuNPs and CuNPs were rapidly synthesized and the formations were observed within 1 h and 24 h, respectively. Further the morphological images of the nanoparticles were confirmed through transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). The high-resolution X-ray diffraction (HR-XRD) analysis of the biosynthesized AuNPs and CuNPs were matched with joint committee on powder diffraction standards (JCPDS) file no of 04-0784 and 89-5899, respectively. A strong prominent Au and Cu signals were observed through energy dispersive spectroscopy (EDS) analysis. Fourier transform infrared spectroscopy (FT-IR) analysis confirmed the responsible phytochemicals for the synthesis of AuNPs and CuNPs. In order to assess the toxic effects of AuNPs and CuNPs, bactericidal activity was performed against few of the test pathogens in which the effective inhibition was observed against Gram-negative bacteria than the Gram-positive bacteria. The mode of action and interaction of nanoparticles were performed on the bacterial pathogens and the results concluded that the interaction of nanoparticles initially initiated on the surface of the cell wall adherence followed by ruptured the cells and caused the cell death. In addition to the antibacterial activity, in vitro embryotoxicity studies were performed against zebrafish embryos and the results confirmed that 200 µg/ml concentration of AuNPs showed the embryotoxicity, whereas 2 µg/ml of CuNPs resulted the embryotoxicity. Furthermore, the morphological anomalies of zebrafish embryos revealed the toxic nature of the synthesized nanoparticles.

Outputs from coupled general circulation models (CGCMs) are used in examining tropical Pacific decadal variability (TPDV) and their relationships with El Niño–Southern Oscillation (ENSO). Herein TPDV is classified as either ENSO-induced TPDV (EIT) or ENSO-like TPDV (ELT), based on their correlations with a decadal modulation index of ENSO amplitude and spatial pattern. EIT is identified by the leading EOF mode of the low-pass filtered equatorial subsurface temperature anomalies and is highly correlated with the decadal ENSO modulation index. This mode is characterized by an east–west dipole structure along the equator. ELT is usually defined by the first EOF mode of subsurface temperature, of which the spatial structure is similar to ENSO. Generally, this mode is insignificantly correlated with the decadal modulation of ENSO. EIT closely interacts with the residuals induced by ENSO asymmetries, both of which show similar spatial structures. On the other hand, ELT is controlled by slowly varying ocean adjustments analogous to a recharge oscillator of ENSO. Both types of TPDV have similar spectral peaks on a decadal-to-interdecadal time scale. Interestingly, the variances of both types of TPDV depend on the strength of connection between El Niño–La Niña residuals and EIT, such that the strong two-way feedback between them enhances EIT and reduces ELT. The strength of the two-way feedback is also related to ENSO variability. The flavors of El Niño–La Niña with respect to changes in the tropical Pacific mean state tend to be well simulated when ENSO variability is larger in CGCMs. As a result, stronger ENSO variability leads to intensified interactive feedback between ENSO residuals and enhanced EIT in CGCMs.

The ocean-atmosphere coupling in the northeastern subtropical Pacific is dominated by a Pacific meridional mode (PMM), which spans between the extratropical and tropical Pacific and plays an important role in connecting extratropical climate variability to the occurrence of El Niño. Analyses of observational data and numerical model experiments were conducted to demonstrate that the PMM (and the subtropical Pacific coupling) experienced a rapid strengthening in the early 1990s and that this strengthening is related to an intensification of the subtropical Pacific high caused by a phase change of the Atlantic multidecadal oscillation (AMO). This PMM strengthening favored the development of more central Pacific (CP)-type El Niño events. The recent shift from more conventional eastern Pacific (EP) to more CP-type El Niño events can thus be at least partly understood as a Pacific Ocean response to a phase change in the AMO.