Researchers have demonstrated mixed associations between acculturative stress and prosocial behaviors (actions intended to benefit others) among Latino/a adolescents and emerging adults. The current study aimed to examine the relations between acculturative stress and Latino/a young adults' prosocial behaviors via familism values and emotion reappraisal. Participants were 1,527 Latino/a college students (M _age = 20.35 years, SD = 3.88; 75.2% women) from universities across the United States. The results demonstrated direct and indirect links between acculturative stress and prosocial behaviors. Specifically, acculturative stress was positively related to familism values, which in turn were positively associated with multiple forms of prosocial behaviors. Additionally, emotion reappraisal was positively associated with specific forms of prosocial behaviors. There was also evidence that familism and emotion reappraisals moderated the associations between acculturative stress and specific forms of prosocial behaviors. Discussion focuses on the interplay of culture-related and emotion-regulation processes associated with Latino/a young adults' positive social outcomes.