Exposure to volatile organic compounds (VOCs) in child care environments has raised a public concern. This study aimed to characterize indoor VOCs in four facilities of Head Start programs in Kansas city, Missouri, investigate seasonal and spatial variations in VOC levels, and assess health risks associated with children's VOC exposure. In total, 49 VOCs including aromatic and aliphatic hydrocarbons, aldehydes, glycol ethers, esters and chlorinated hydrocarbons were identified and quantified in the facilities. Significant differences were noted for the VOC concentrations among the facilities. Toluene was the most abundant aromatic hydrocarbon detected in all the air samples, with a narrow median concentration range of 2.17–3.07 μg/m3. 2-(2-Methoxyethoxy)ethanol never reported in prior research was detected in only one facility in this study, with the median (range) concentration of 0.83 (