- Ding, Yichen;
- Lee, Juhyun;
- Ma, Jianguo;
- Sung, Kevin;
- Yokota, Tomohiro;
- Singh, Neha;
- Dooraghi, Mojdeh;
- Abiri, Parinaz;
- Wang, Yibin;
- Kulkarni, Rajan P;
- Nakano, Atsushi;
- Nguyen, Thao P;
- Fei, Peng;
- Hsiai, Tzung K
Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.