Copper is an essential micronutrient for plant growth. It is an important component of proteins. In the present research the effect of copper toxicity on chlorophyll, sugar, protein content and antioxidant enzymes activity in both mycorrhizal and non-mycorrhizal tomato plants were studied. The experiment was performed by using two treatments (mycorrhizal and non-mycorrhizal) and five concentrations of CuSo4 solution added to Hogland nutrient solution . Copper toxicity caused reduction in growth in all treatment (non-AM). It may be due to accumulation of Cu in leaves. Total sugar content in both shoot and roots, the total chlorophyll content in leaves, Protein content in shoots and roots, antioxidant enzyme activity and root colonization were determined.