The neuronal lineage of the olfactory epithelium (OE) is a cell lineage that includes the neuronal stem cell and its progeny (ultimately the mature olfactory receptor neuron [ORN]). Recent studies, including further characterization of the neuronal lineage of the OE, and of factors that influence proliferation, survival, and death of cells of this lineage, have contributed significantly to understanding of neuronal homeostasis, i.e., normal maintenance of neuronal number, in mammalian OE. Our recent studies indicate that in adult mice, all cell types of the neuronal lineage of the OE-neuronal precursors, immature ORNs and mature ORNs-undergo constitutive death, i.e., a normal, basal level of cell death, that is characteristic of programmed cell death or apoptosis. To some extent, constitutive cell death in this lineage may reflect random environmental insults; however, this may also be the result of an ongoing developmental program that acts to control both numbers and phenotypic organization of olfactory neurons. Although a variety of extrinsic and intrinsic factors are likely to contribute to cell death in the neuronal lineage of the OE, most have not been thoroughly studied. Detailed analysis of one of these factors, effects of target deprivation, suggests that survival of individual cell types of the neuronal lineage of the OE may be differentially regulated with mature ORNs, but not immature ORNs or neuronal precursors, dependent upon the olfactory bulb for their survival. Factors normally provided to cells of the ORN lineage, as in other neuronal systems, are likely to promote survival by inhibiting an endogenous genetic program of cell death. Whether candidate polypeptide growth factors, e.g., the neurotrophins, or other pharmacological inhibitors ofapoptosis will eventually play a role in the treatment of specific anosmias remains to be deter-mined.

Methane (CH4) emissions from natural landscapes constitute roughly half of global CH4 contributions to the atmosphere, yet large uncertainties remain in the absolute magnitude and the seasonality of emission quantities and drivers. Eddy covariance (EC) measurements of CH4 flux are ideal for constraining ecosystem-scale CH4 emissions due to quasi-continuous and high-temporal-resolution CH4 flux measurements, coincident carbon dioxide, water, and energy flux measurements, lack of ecosystem disturbance, and increased availability of datasets over the last decade. Here, we (1) describe the newly published dataset, FLUXNET-CH4 Version 1.0, the first open-source global dataset of CH4 EC measurements (available at https://fluxnet.org/data/fluxnet-ch4-community-product/, last access: 7 April 2021). FLUXNET-CH4 includes half-hourly and daily gap-filled and non-gap-filled aggregated CH4 fluxes and meteorological data from 79 sites globally: 42 freshwater wetlands, 6 brackish and saline wetlands, 7 formerly drained ecosystems, 7 rice paddy sites, 2 lakes, and 15 uplands. Then, we (2) evaluate FLUXNET-CH4 representativeness for freshwater wetland coverage globally because the majority of sites in FLUXNET-CH4 Version 1.0 are freshwater wetlands which are a substantial source of total atmospheric CH4 emissions; and (3) we provide the first global estimates of the seasonal variability and seasonality predictors of freshwater wetland CH4 fluxes. Our representativeness analysis suggests that the freshwater wetland sites in the dataset cover global wetland bioclimatic attributes (encompassing energy, moisture, and vegetation-related parameters) in arctic, boreal, and temperate regions but only sparsely cover humid tropical regions. Seasonality metrics of wetland CH4 emissions vary considerably across latitudinal bands. In freshwater wetlands (except those between 20g g€¯S to 20g g€¯N) the spring onset of elevated CH4 emissions starts 3g€¯d earlier, and the CH4 emission season lasts 4g€¯d longer, for each degree Celsius increase in mean annual air temperature. On average, the spring onset of increasing CH4 emissions lags behind soil warming by 1 month, with very few sites experiencing increased CH4 emissions prior to the onset of soil warming. In contrast, roughly half of these sites experience the spring onset of rising CH4 emissions prior to the spring increase in gross primary productivity (GPP). The timing of peak summer CH4 emissions does not correlate with the timing for either peak summer temperature or peak GPP. Our results provide seasonality parameters for CH4 modeling and highlight seasonality metrics that cannot be predicted by temperature or GPP (i.e., seasonality of CH4 peak). FLUXNET-CH4 is a powerful new resource for diagnosing and understanding the role of terrestrial ecosystems and climate drivers in the global CH4 cycle, and future additions of sites in tropical ecosystems and site years of data collection will provide added value to this database. All seasonality parameters are available at 10.5281/zenodo.4672601 (Delwiche et al., 2021). Additionally, raw FLUXNET-CH4 data used to extract seasonality parameters can be downloaded from https://fluxnet.org/data/fluxnet-ch4-community-product/ (last access: 7 April 2021), and a complete list of the 79 individual site data DOIs is provided in Table 2 of this paper.