Benthic invertebrates are vital not only for the place they hold in the trophic web of the marine ecosystem, but also for the incredible diversity that they add to the world. This is especially true of the dorid nudibranchs (family Dorididae), a group of specialist predators that are also the most diverse family in a clade of shell-less gastropods. Little work has been done on the roles that environment and behavior play on distribution patterns of dorid nuidbranchs. By carrying out habitat surveys, I found that two species of dorid nudibranchs (Chromodoris lochiandChromodorissp.) occupy different habitats in Cook’s Bay. Behavioral interaction tests showed that both species orient more reliably toward conspecifics than toward allospecifics.C. lochihas a greater propensity to aggregate thanChromodorissp. These findings indicated that the distribution patterns are a result of both habitat preference and aggregation behaviors. Further inquiry into these two areas is needed to make additional conclusions on the forces driving distribution. Information in this area is necessary to inform future conservation decisions. 

In 2016, we undertook a nationally representative wind power perceptions survey of individuals living within 8 km of over 600 projects in the United States, generating 1705 telephone, web, and mail responses. We sought information on a variety of topics, including procedural fairness and its relationship to project attitude, the foci of the present analysis. We present a series of descriptive statistics and regression results, emphasizing those residents who were aware of their local project prior to construction. Sample weighting is employed to account for stratification and non-response. We find that a developer being open and transparent, a community being able to influence the outcome, and having a say in the planning process are all statistically significant predictors of a process perceived as being ‘fair,’ with an open and transparent developer having the largest effect. We also find developer transparency and ability to influence outcomes to have statistically significant relationships to a more positive attitude, with those findings holding when aesthetics, landscape, and wind turbine sound considerations are controlled for. The results indicate that jurisdictions might consider developing procedures, which ensure citizens are consulted and heard, and benchmarks or best practices for developer interaction with communities and citizens.

Nuclear energy provides a widely applied carbon-reduced energy source. Following operation, the spent nuclear fuel (SNF), containing a mixture of radiotoxic elements such as transuranics, needs to be safely disposed of. Safe storage of SNF in a deep geological repository (DGR) relies on multiple engineered and natural retention barriers to prevent environmental contamination. In this context, zirconia (ZrO2) formed on the SNF rod cladding, could be employed as an engineered barrier for immobilization of radionuclides via structural incorporation. This study investigates the incorporation of Eu3+ and Cm3+, representatives for trivalent transuranics, into zirconia by co-precipitation and crystallization in aqueous solution at 80 °C. Complementary structural and microstructural characterization has been carried out by powder X-ray diffraction (PXRD), spectrum imaging analysis based on energy-dispersive X-ray spectroscopy in scanning transmission electron microscopy mode (STEM-EDXS), and luminescence spectroscopy. The results reveal the association of the dopants with the zirconia particles and elucidate the presence of distinct bulk and superficially incorporated species. Hydrothermal aging for up to 460 days in alkaline media points to great stability of these incorporated species after initial crystallization, with no indication of phase segregation or release of Eu3+ and Cm3+ over time. These results suggest that zirconia would be a suitable technical retention barrier for mobilized trivalent actinides in a DGR.

As wind turbines and the number of wind projects scale throughout the world, a growing number of individuals might be affected by these structures. For some people, wind turbine sounds and their effects on the landscape can be annoying and could even prompt stress reactions. This comparative study analyzed a combined sample of survey respondents from the U.S., Germany and Switzerland. It utilized a newly developed assessment scale (AS-Scale) to reliably characterize these stress-impacted individuals living within populations near turbines. Findings indicate low prevalence of annoyance, stress symptoms and coping strategies. Noise annoyance stress (NAS-Scale) was negatively correlated with the perceptions of a lack of fairness of the wind project's planning and development process, among other subjective variables. Objective indicators, such as the distance from the nearest turbine and sound pressure level modeled for each respondent, were not found to be correlated to noise annoyance. Similar result patterns were found across the European and U.S. samples.

Structural studies on G protein-coupled receptors (GPCRs) provide important insights into the architecture and function of these important drug targets. However, the crystallization of GPCRs in active states is particularly challenging, requiring the formation of stable and conformationally homogeneous ligand-receptor complexes. Native hormones, neurotransmitters, and synthetic agonists that bind with low affinity are ineffective at stabilizing an active state for crystallogenesis. To promote structural studies on the pharmacologically highly relevant class of aminergic GPCRs, we here present the development of covalently binding molecular tools activating Gs-, Gi-, and Gq-coupled receptors. The covalent agonists are derived from the monoamine neurotransmitters noradrenaline, dopamine, serotonin, and histamine, and they were accessed using a general and versatile synthetic strategy. We demonstrate that the tool compounds presented herein display an efficient covalent binding mode and that the respective covalent ligand-receptor complexes activate G proteins comparable to the natural neurotransmitters. A crystal structure of the β2-adrenoreceptor in complex with a covalent noradrenaline analog and a conformationally selective antibody (nanobody) verified that these agonists can be used to facilitate crystallogenesis.

With results from a nationwide survey sponsored by the U.S. Department of Energy, factors that affect outdoor audibility and noise annoyance of wind turbines were evaluated. Wind turbine and summer daytime median background sound levels were estimated for 1043 respondents. Wind turbine sound level was the most robust predictor of audibility yet only a weak, albeit significant, predictor of noise annoyance. For each 1 dB increase in wind turbine sound level (L_1h-max), the odds of hearing a wind turbine on one's property increased by 31% [odds ratio (OR): 1.31; 95% CI (confidence interval): 1.25-1.38] and the odds of moving to the next level of annoyance increased by 9% (OR: 1.09; 95% CI: 1.02-1.16). While audibility was overwhelmingly dependent on turbine sound level, noise annoyance was best explained by visual disapproval (OR: 11.0; 95% CI: 4.8-25.4). The final models correctly predict audibility and annoyance level for 80% and 62% of individuals, respectively. The results demonstrate that among community members not receiving personal benefits from wind projects, the Community Tolerance Level of wind turbine noise for the U.S. aligns with the international average, further supporting observations that communities are less tolerant of wind turbine noise than other common environmental noise sources at equivalent A-weighted sound levels.

Long noncoding RNAs (lncRNAs) are a heterogenous group of RNAs, which can encode small proteins. The extent to which developmentally regulated lncRNAs are translated and whether the produced microproteins are relevant for human development is unknown. Using a human embryonic stem cell (hESC)-based pancreatic differentiation system, we show that many lncRNAs in direct vicinity of lineage-determining transcription factors (TFs) are dynamically regulated, predominantly cytosolic, and highly translated. We genetically ablated ten such lncRNAs, most of them translated, and found that nine are dispensable for pancreatic endocrine cell development. However, deletion of LINC00261 diminishes insulin⁺ cells, in a manner independent of the nearby TF FOXA2. One-by-one disruption of each of LINC00261's open reading frames suggests that the RNA, rather than the produced microproteins, is required for endocrine development. Our work highlights extensive translation of lncRNAs during hESC pancreatic differentiation and provides a blueprint for dissection of their coding and noncoding roles.