California must operate and maintain an effective and efficient transportation infrastructure while ensuring that the health of communities and the planet are not compromised. By assessing transportation projects using a life-cycle perspective, all relevant emission sources and activities from the construction, operation, maintenance, and end-of-life phases can be analyzed and mitigated. This report presents a framework to assess the life-cycle human health and climate change impacts from six types of transportation projects: (1) Roadways; (2) Marine ports; (3) Logistical distribution centers; (4) Railyards; (5) Bridges and overpasses; and (6) Airports. The framework was applied using an integrated model to assess fine particulate matter (PM2.5) and greenhouse gas (GHG) emissions, noise impacts, and monetized damages (Value of Statistical Life, Social Cost of Carbon) from two case studies: routine resurfacing and vehicle operations on road segments within the San Francisco Bay Area using 2019 data, and annual marine, cargo, rail, trucking, and infrastructure maintenance operations at the Port of Oakland in 2020. The results suggest that emission sources in a project’s supply chain and construction (material production and deliveries, construction activities, fuel refining) can significantly contribute to the full scope of impacts from transportation systems. Equitable mitigation policies (e.g., electrification, pollution control technologies) need to be tailored to address the sources that impact communities the most.