A truss structure was recently introduced as an interbody fusion cage. As a truss system, some of the connected elements may be in a state of compression and others in tension. This study aimed to quantify both the mean and variance of strut strains in such an implant when loaded in a simulated fusion condition with vertebral body or contoured plastic loading platens ex vivo. Cages were each instrumented with 78 fiducial spheres, loaded between platens (vertebral body or contoured plastic), imaged using high resolution micro-CT, and analyzed for deformation and strain of each of the 221 struts. With repeated loading of a cage by vertebral platens, the distribution (variance, indicated by SD) of strut strains widened from 50N control (4±114με, mean±SD) to 1000N (-23±273με) and 2000N (-48±414με), and between 1000N and 2000N. With similar loading of multiple cages, the strain distribution at 2000N (23±389με) increased from 50N control. With repeated loading by contoured plastic platens, induced strains at 2000N had a distribution similar to that induced by vertebral platens (84±426με). In all studies, cages exhibited increases in strut strain amplitude when loaded from 50N to 1000N or 2000N. Correspondingly, at 2000N, 59-64% of struts exhibited strain amplitudes consistent with mechanobiologically-regulated bone homeostasis. At 2000N, vertically-oriented struts exhibited deformation of -2.87±2.04μm and strain of -199±133με, indicating overall cage compression. Thus, using an ex vivo 3-D experimental biomechanical analysis method, a truss implant can have strains induced by physiological loading that are heterogeneous and of amplitudes consistent with mechanobiological bone homeostasis.