Numerical simulations have shown that the network fundamental diagram (NFD) of a signalized network is significantly affected by the green ratio. An analytical approximation of the NFD has been derived from the link transmission model. However, the consistency between these approaches has not been established, and the impacts of other factors are still unrevealed. This research evalutes the impacts of start-up and clearance behaviors in a signalized network from a network fundamental diagram approach. Microscopic simulations based on Newell’s car-following model are used for testing the bounded acceleration (start-up) and aggressiveness (clearance) effects on the shape of the NFD in a signalized ring road. This new approach is shown to be consistent with theoretical results from the link transmission model, when the acceleration is unbounded and vehicles have the most aggressive clearance behaviors. This consistency validates both approaches; but the link transmission model cannot be easily extended to incorporate more realistic start-up or clearance behaviors. With the new approach, this project demonstrates that both bounded acceleration and different aggressiveness lead to distinct network capacities and fundamental diagrams. In particular, they lead to start-up and clearance lost times of several seconds; and these lost times are additive. Therefore, the important role that these behaviors play in the NFD shape is studied to reach a better understanding of how the NFD responds to changes. This will help with designing better start-up and clearance behaviors for connected and autonomous vehicles.