Purpose: The goal of this study was to develop an immunodeficient rat model of retinal degeneration (RD nude rats) that will not reject transplanted human cells. Methods: SD-Tg(S334ter)3Lav females homozygous for a mutated mouse rhodopsin transgene were mated with NTac:NIH-Whn (NIH nude) males homozygous for the Foxn1 rnu allele. Through selective breeding, a new stock, SD-Foxn1 Tg(S334ter)3Lav (RD nude) was generated such that all animals were homozygous for the Foxn1 rnu allele and either homo- or hemizygous for the S334ter transgene. PCR-based assays for both the Foxn1 rnu mutation and the S334ter transgene were developed for accurate genotyping. Immunodeficiency was tested by transplanting sheets of hESC-derived neural progenitor cells to the subretinal space of RD nude rats, and, as a control, NIH nude rats. Rats were killed between 8 and 184 days after surgery, and eye sections were analyzed for human, neuronal, and glial markers. Results: After transplantation to RD nude and to NIH nude rats, hESC-derived neural progenitor cells differentiated to neuronal and glial cells, and migrated extensively from the transplant sheets throughout the host retina. Migration was more extensive in RD nude than in NIH nude rats. Already 8 days after transplantation, donor neuronal processes were found in the host inner plexiform layer. In addition, host glial cells extended processes into the transplants. The host retina showed the same photoreceptor degeneration pattern as in the immunocompetent SD-Tg(S334ter)3Lav rats. Recipients survived well after surgery. Conclusions: This new rat model is useful for testing the effect of human cell transplantation on the restoration of vision without interference of immunosuppression. © 2014 Springer-Verlag.

Background: Measurements of exclusive meson production are a useful tool in the study of hadronic structure. In particular, one can discern the relevant degrees of freedom at different distance scales through these studies. Purpose: To study the transition between nonperturbative and perturbative quantum chromodynamics as the square of four-momentum transfer to the struck proton, -t, is increased. Method: Cross sections for the H1(e,e′π+)n reaction were measured over the -t range of 0.272 to 2.127 GeV2 with limited azimuthal coverage at fixed beam energy of 4.709 GeV, Q2 of 2.4 GeV2, and W of 2.0 GeV at the Thomas Jefferson National Accelerator Facility (JLab) Hall C. Results: The -t dependence of the measured π+ electroproduction cross section generally agrees with prior data from JLab Halls B and C. The data are consistent with a Regge amplitude-based theoretical model but show poor agreement with a generalized parton distribution-based model. Conclusion: The agreement of cross sections with prior data implies small contribution from the interference terms, and the confirmation of the change in t slopes between the low- and high - t regions previously observed in photoproduction indicates the changing nature of the electroproduction reaction in our kinematic regime.

Backward-angle meson electroproduction above the resonance region, which was previously ignored, is anticipated to offer unique access to the three quark plus sea component of the nucleon wave function. In this Letter, we present the first complete separation of the four electromagnetic structure functions above the resonance region in exclusive ω electroproduction off the proton, ep→e^{'}pω, at central Q^{2} values of 1.60, 2.45  GeV^{2}, at W=2.21  GeV. The results of our pioneering -u≈-u_{min} study demonstrate the existence of a unanticipated backward-angle cross section peak and the feasibility of full L/T/LT/TT separations in this never explored kinematic territory. At Q^{2}=2.45  GeV^{2}, the observed dominance of σ_{T} over σ_{L}, is qualitatively consistent with the collinear QCD description in the near-backward regime, in which the scattering amplitude factorizes into a hard subprocess amplitude and baryon to meson transition distribution amplitudes: universal nonperturbative objects only accessible through backward-angle kinematics.

Inclusive electron scattering from nuclear targets has been measured to extract the nuclear dependence of the inelastic cross section (σA) in Hall C at the Thomas Jefferson National Accelerator facility. Results are presented for H2, He3, He4, B9, C12, Cu63, and Au197 at an incident electron beam energy of 5.77 GeV for a range of momentum transfer from Q2 = 2 to 7 (GeV/c)2. These data improve the precision of the existing measurements of the EMC effect in the nuclear targets at large x and allow for more detailed examinations of the A dependence of the EMC effect.

We present extractions of the nucleon nonsinglet moments utilizing new precision data on the deuteron F_{2} structure function at large Bjorken-x determined via the Rosenbluth separation technique at Jefferson Lab Experimental Hall C. These new data are combined with a complementary set of data on the proton previously measured in Hall C at similar kinematics and world datasets on the proton and deuteron at lower x measured at SLAC and CERN. The new Jefferson Lab data provide coverage of the upper third of the x range, crucial for precision determination of the higher moments. In contrast to previous extractions, these moments have been corrected for nuclear effects in the deuteron using a new global fit to the deuteron and proton data. The obtained experimental moments represent an order of magnitude improvement in precision over previous extractions using high x data. Moreover, recent exciting developments in lattice QCD calculations provide a first ever comparison of these new experimental results with calculations of moments carried out at the physical pion mass, as well as a new approach that first calculates the quark distributions directly before determining moments.

The H1(e,e′K+)Λ reaction was studied as a function of the Mandelstam variable -t using data from the E01-004 (FPI-2) and E93-018 experiments that were carried out in Hall C at the 6 GeV Jefferson Laboratory. The cross section was fully separated into longitudinal and transverse components, and two interference terms at four-momentum transfers Q2 of 1.00, 1.36, and 2.07GeV2. The kaon form factor was extracted from the longitudinal cross section using the Regge model by Vanderhaeghen et al. [Phys. Rev. C 57, 1454 (1998)PRVCAN0556-281310.1103/PhysRevC.57.1454]. The results establish the method, previously used successfully for pion analyses, for extracting the kaon form factor. Data from 12 GeV Jefferson Laboratory experiments are expected to have sufficient precision to distinguish between theoretical predictions, for example, recent perturbative QCD calculations with modern parton distribution amplitudes. The leading-twist behavior for light mesons is predicted to set in for values of Q2 between 5 and 10GeV2, which makes data in the few-GeV regime particularly interesting. The Q2 dependence at fixed x and -t of the longitudinal cross section that we extracted seems consistent with the QCD factorization prediction within the experimental uncertainty.

The GEp-III and GEp-2γ experiments, carried out in Jefferson Lab's Hall C from 2007–2008, consisted of measurements of polarization transfer in elastic electron–proton scattering at momentum transfers of Q2=2.5,5.2,6.8, and 8.54 GeV 2. These measurements were carried out to improve knowledge of the proton electromagnetic form factor ratio R=μpGEp∕GMp at large values of Q2 and to search for effects beyond the Born approximation in polarization transfer observables at Q2=2.5GeV2. The final results of both experiments were reported in a recent archival publication. A full reanalysis of the data from both experiments was carried out in order to reduce the systematic and, for the GEp-2γ experiment, statistical uncertainties. This technical note provides additional details of the final analysis omitted from the main publication, including the final evaluation of the systematic uncertainties.

The nuclear dependence of the inclusive inelastic electron scattering cross section (the EMC effect) has been measured for the first time in B10 and B11. Previous measurements of the EMC effect in A≤12 nuclei showed an unexpected nuclear dependence; B10 and B11 were measured to explore the EMC effect in this region in more detail. Results are presented for Be9, B10, B11, and C12 at an incident beam energy of 10.6 GeV. The EMC effect in the boron isotopes was found to be similar to that for Be9 and C12, yielding almost no nuclear dependence in the EMC effect in the range A=4-12. This represents important new data supporting the hypothesis that the EMC effect depends primarily on the local nuclear environment due to the cluster structure of these nuclei.

Subsequent to the release of our original paper, we discovered in the context of preparing our technical supplement [1] for journal publication that a typographical error had existed in the text file that the analysis program used to construct the beam polarization "database" for both the original analysis, published in Ref. [2], and our final analysis. The electron-beam polarization P e and the analyzing power A y cancel exactly in the ratio R , which is proportional to the ratio P t / P l of the transferred polarization components. On the other hand, the extraction of the relative e dependence of P l / P Born l relies on knowledge of the beam polarization. As such, data taking was interrupted roughly every two days during the GEp - 2 ? experiment to perform invasive measurements of the beam polarization using the Hall C Moller polarimeter [3]. The run range affected by the typographical error was entirely contained within the data collected at Q 2 = 2.5 GeV 2 with a beam energy of E e = 3.680 GeV during January 2008. The data from this configuration were combined with the data collected at E e = 3.548 GeV due to the nearly complete overlap of these two settings in terms of Q 2 and e acceptance. It is worth remarking that this typographical error went unnoticed for so long because it only affected a small fraction of the data (less than half of the combined data for ? e ? = 0.790 ) and the difference between the actually assigned beam polarization and the polarization that should have been assigned was comparable in magnitude to the point-to-point systematic uncertainty of the measurement itself. As such, its effect did not show up in various diagnostic plots and statistical tests, such as the time stability of the extracted P l / P Born l ratio. The data for both E e = 3.548 and E e = 3.680 GeV were reprocessed using the corrected beam polarizations to determine the effect of the typographical error on the combined physics results at ? e ? = 0.790 . Because the value of P e cancels in the ratio R , changes in the assumed beam polarization can only affect the results for R via statistical fluctuations due to changes in the relative weighting of different run ranges in the unbinned maximum-likelihood estimators for R . These effects are negligible on the scale of both the statistical and the systematic uncertainties of the data. More noticeable changes are expected in the ratio P l / P Born l since the extracted value of P l is inversely proportional to the assumed value of P e . Table I shows the effect of the corrected beam polarization database on the polarization transfer observables for the combined data for the ? e ? = 0.790 setting, the only measurement affected by the typographical error. The analyzing power did not need to be recalibrated since it was determined using the ? e ? = 0.153 data, which were not affected by the typographical error. As expected, the change in the ratio R is negligible. The value of P Born l , which is computed event by event from the global fit described in the Appendix of the original paper and does not depend on P e , is also unchanged. The magnitudes of P t , P l , and P l / P Born l are reduced by a common multiplicative factor, reflecting the fact that the beam polarization had been underestimated for the run range affected by the typographical error. The most important result of the corrected analysis is that the ratio P l / P Born l has decreased by 0.0024 from 1.0167 to 1.0143, a change comparable in magnitude to the statistical uncertainty but small compared to the total and point-to-point systematic uncertainties. The P l / P Born l result for the original publication [2] would be reduced by the same multiplicative factor as the final result. The physics conclusions of both publications are not materially changed by this correction. (Table Presented). (Figure Presented).

Structure functions, as measured in lepton-nucleon scattering, have proven to be very useful in studying the partonic dynamics within the nucleon. However, it is experimentally difficult to separately determine the longitudinal and transverse structure functions, and consequently there are substantially less data available in particular for the longitudinal structure function. Here, we present separated structure functions for hydrogen and deuterium at low four-momentum transfer squared, Q2<1GeV2, and compare them with parton distribution parametrization and kT factorization approaches. While differences are found, the parametrizations generally agree with the data, even at the very low-Q2 scale of the data. The deuterium data show a smaller longitudinal structure function and a smaller ratio of longitudinal to transverse cross section, R, than the proton. This suggests either an unexpected difference in R for the proton and the neutron or a suppression of the gluonic distribution in nuclei.