This paper has three goals: (i) to present a partial description of the intricate semantic selectional restrictions on the noun phrases in what we call here the Causal Have Construction (CHC), (ii) to show that four and five-year old children are sensitive to these selectional restrictions without much exposure to CHCs, and (iii) to discuss some implications of these findings for theories of language and language acquisition. Our interest in this topic derives from the possibilities it opens up for a deeper understanding of the organization of the mental structures that give rise to these semantic selectional facts, an understanding which we believe implicates an intricate and nontrivial interaction between grammatical and conceptual knowledge.

Cytology of sparingly available cell samples from both clinical and experimental settings would benefit from high-selectivity protein tools. To minimize cell handling losses in sparse samples, we design a multi-stage assay using a lab-on-a-disc that integrates cell handling and subsequent single-cell western blotting (scWestern). As the two-layer microfluidic device rotates, the induced centrifugal force directs dissociated cells to dams, which in turn localize the cells over microwells. Cells then sediment into the microwells, where the cells are lysed and subjected to scWestern. Taking into account cell losses from loading, centrifugation, and lysis-buffer exchange, our lab-on-a-disc device handles cell samples with as few as 200 cells with 75% cell settling efficiencies. Over 70% of microwells contain single cells after the centrifugation. In addition to cell settling efficiency, cell-size filtration from a mixed population of two cell lines is also realized by tuning the cell time-of-flight during centrifugation (58.4% settling efficiency with 6.4% impurity). Following the upstream cell handling, scWestern analysis detects four proteins (GFP, β-TUB, GAPDH, and STAT3) in a glioblastoma cell line. By integrating the lab-on-a-disc cell preparation and scWestern analysis, our platform measures proteins from sparse cell samples at single-cell resolution.