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Open Access Publications from the University of California

Scholarly Works (3 results)

  • Article
  • Peer Reviewed
LBL Publications (2005)
    Cover page: NMR structure of hypothetical protein MG354 from Mycoplasma genitalium
    • Article
    • Peer Reviewed
    LBL Publications (2005)

    The crystal structure of the hypothetical protein MPN555 from Mycoplasma pneumoniae (gi pbar 1673958) has been determined to a resolution of 2.8 Angstrom using anomalous diffraction data at the Se peak wavelength. Structure determination revealed a mostly alpha-helical protein with a three-lobed shape. The three lobes or fingers delineate a central binding groove and additional grooves between lobes 1 and 3, and between lobes 2 and 3. For one of the molecules in the asymmetric unit, the central binding pocket was filled with a peptide from the uncleaved N-terminal affinity tag. The MPN555 structure has structural homology to two bacterial chaperone proteins, SurA and trigger factor from Escherichia coli. The structural data and the homology to other chaperone for MPN555.

      Cover page: Structure of the hypothetical Mycoplasma protein, MPN555, suggests a chaperone function
      • Article
      • Peer Reviewed
      UC Santa Cruz Previously Published Works (2008)
      We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures.
        Cover page: Capturing Hammerhead Ribozyme Structures in Action by Modulating General Base Catalysis
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