Amphiphilic polymer conetworks were prepared, characterized and used as highly activating matrices for phase transfer reactions. Several applications such as biocatalysis in organic solvents, metathesis reactions in water, biosensor designs for detecting metabolites in organic solvents, as well as gas sensor designs are discussed. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.