In many advanced low NOx gas turbine combustion techniques, such as rich-burn-quick-mix, lean-burn (RQL), jet mixing in a reacting, hot, fuel-rich crossflow plays an important role in minimizing all pollutant emissions and maximizing combustion efficiency. Assessing the degree of mixing and predicting jet penetration is critical to the optimization of the jet injection design strategy. Different passive scalar quantities, including carbon, oxygen, and helium, are compared to quantify mixing in an atmospheric RQL combustion rig under reacting conditions. The results show that the O2 based jet mixture fraction underpredicts the C-based mixture fraction due to jet dilution and combustion, whereas the He tracer overpredicts it possibly due to differences in density and diffusivity. The He method also exhibits significant scatter in the mixture fraction data that can most likely be attributed to differences in gas density and turbulent diffusivity. The jet mixture fraction data were used to evaluate planar spatial unmixedness, which showed good agreement for all three scalars. This investigation suggests that, with further technique refinement, either O2 or a He tracer could be used instead of C to determine the extent of reaction and mixing in an RQL combustor.