Elevated indoor CO₂ levels are indicative of insufficient ventilation in occupied spaces and correlate with elevated concentrations of pollutants of indoor origin. Adverse health and well-being outcomes associated with elevated indoor CO₂ levels are based on CO2 as a proxy, although some emerging evidence suggests CO₂ itself may impact human cognition. Using portable monitors, we conducted an exposure study with 16 subjects in Singapore to understand the levels, dynamics and influencing factors of personal exposure to CO₂. Participants carried a CO₂ monitor continuously for 7-day periods recording their exposure levels at 1-min intervals. A recall diary was maintained of time-microenvironment-activity budget.  We found that the mode of bedroom ventilation was a major determinant of CO₂ exposure. Approximately half of the participants slept in bedrooms employing ductless split air-conditioners (group “AC”); half slept in bedrooms naturally ventilated through operable windows (group “NV”).  Median CO₂ exposure levels for AC vs. NV groups are significantly different (  = 650 ppm vs.  = 550 ppm, p < 0.001). Mean daily integrated exposures for group AC were statistically higher than for group NV: 22,800 ppm h/d vs. 16,000 ppm h/d (p < 0.005). Exposure events associated with potential adverse cognitive implications (duration > 2.5 h, average CO₂ mixing ratio > 1000 ppm) occurred, on average, at frequencies of 0.5 d^-1 across all participants, 0.6 d^-1 for AC participants and 0.2 d^-1 for NV participants. The majority of such events occurred in the home (86%), followed by work (9%) and transit (3%).

Introduction

Biological particles deposit on air handling system filters as they process air. This study reports and interprets abundance and diversity information regarding biomass accumulation on ordinarily used filters acquired from several locations in a university environment.

Methods

DNA-based analysis was applied both to quantify (via DNA fluorometry and qPCR) and to characterize (via high-throughput sequencing) the microbial material on filters, which mainly processed recirculated indoor air. Results were interpreted in relation to building occupancy and ventilation system operational parameters.

Results

Based on accumulated biomass, average DNA concentrations per AHU filter surface area across nine indoor locations after twelve weeks of filter use were in the respective ranges 1.1 to 41 ng per cm2 for total DNA, 0.02 to 3.3 ng per cm2 for bacterial DNA and 0.2 to 2.0 ng DNA per cm2 for fungal DNA. The most abundant genera detected on the AHU filter samples were Clostridium, Streptophyta, Bacillus, Acinetobacter and Ktedonobacter for bacteria and Aspergillus, Cladosporium, Nigrospora, Rigidoporus and Lentinus for fungi. Conditional indoor airborne DNA concentrations (median (range)) were estimated to be 13 (2.6-107) pg/m3 for total DNA, 0.4 (0.05-8.4) pg/m3 for bacterial DNA and 2.3 (1.0-5.1) pg/m3 for fungal DNA.

Conclusion

Conditional airborne concentrations and the relative abundances of selected groups of genera correlate well with occupancy level. Bacterial DNA was found to be more responsive than fungal DNA to differences in occupancy level and indoor environmental conditions.

Introduction

Ventilation system filters process recirculated indoor air along with outdoor air. This function inspires the idea of using the filter as an indoor bioaerosol sampler. While promising, there remains a need to investigate several factors that could limit the accuracy of such a sampling approach. Among the important factors are the dynamics of microbial assemblages on filter surfaces over time and the differential influence of outdoor versus recirculated indoor air.

Methods

This study collected ventilation system filter samples from an air handling unit on a regular schedule over a 21-week period and analyzed the accumulation patterns of biological particles on the filter both quantitatively (using fluorometry and qPCR) and in terms of microbial diversity (using 16S rDNA and ITS sequencing).

Results

The quantitative result showed that total and bacterial DNA accumulated monotonically, rising to 41 ng/cm2 for total DNA and to 2.8 ng/cm2 for bacterial DNA over the 21-week period. The accumulation rate of bacterial DNA correlated with indoor occupancy level. Fungal DNA first rose to 4.0 ng/cm2 before showing a dip to 1.4 ng/cm2 between weeks 6 and 10. The dip indicated a possible artifact of this sampling approach for quantitative analysis as DNA may not be conserved on the filter over the months-long service period. The sequencing results indicate major contributions from outdoor air for fungi and from recirculated indoor air for bacteria. Despite the quantitative changes, the community structure of the microbial assemblages was stable throughout the 21-week sampling period, highlighting the robustness of this sampling method for microbial profiling.

Conclusion

This study supports the use of ventilation system filters as indoor bioaerosol samplers, but with caveats: 1) an outdoor reference is required to properly understand the contribution of outdoor bioaerosols; and 2) there is a need to better understand the persistence and durability of the targeted organisms on ventilation system filters.

This study is concerned with the role of a fin-and-tube heat exchanger in modifying microbial indoor air quality. Specifically, depositional losses of ambient bioaerosols and particles onto dry (not cooled) and wet (cool) coil surfaces were measured for different airspeeds passing through the test coil. Total, bacterial and fungal DNA concentrations in condensate water produced by a wet coil were also quantified by means of fluorescent dsDNA-binding dye and qPCR assays. Results revealed that the deposition of bioaerosols and total particles is substantial on coil surfaces, especially when wet and cool. The average deposition fraction was 0.14 for total DNA, 0.18 for bacterial DNA and 0.22 for fungal DNA on the dry coil, increasing to 0.51 for total DNA, 0.50 for bacterial DNA and 0.68 for fungal DNA on the wet coil. Overall, as expected, deposition fractions increased with increasing particle size and increasing airspeed. Deposited DNA was removed from the cooling coil surfaces through the flow of condensing water at a rate comparable to the rate of direct deposition from air. A downward trend of bacterial and fungal DNA measured in condensate water over time provides suggestive evidence of biological growth on heat exchangers during nonoperational times of a ventilation system. This investigation provides new information about bioaerosol deposition onto a conventional fin-and-tube cooling coil, a potentially important factor influencing indoor exposure to microbial aerosols in air-conditioned buildings.