Inbred rats from the Roman low-avoidance strain (RLA-I), but not from the Roman high-avoidance strain (RHA-I) increased preference for ethanol after being exposed to sessions of appetitive extinction (Manzo et al. Physiol Behav 2014 123:86-92). RLA-I rats have shown greater sensitivity than RHA-I rats to a variety of anxiogenic situations, including those involving reward loss. Such increased fluid preference did not occur after acquisition (reinforced) sessions or in control groups with postsession access to water, rather than ethanol. Because ethanol has anxiolytic properties in tasks involving reward loss, oral consumption after extinction sessions was interpreted as anti-anxiety or emotional self-medication (ESM). The present research was an attempt to reduce or eliminate the ESM effect in RLA-I rats by giving them 50% partial reinforcement training during the acquisition of an instrumental response, a treatment known to induce resilience to loss-induced anxiety. As expected, partially reinforced RLA-I rats showed a higher resistance to extinction in comparison to continuously reinforced animals, displaying lower ethanol consumption than continuously reinforced rats during the postsession preference test. Partial and continuous control groups receiving water during the preference tests showed no changes in preference. These results suggest that exposure to reward uncertainty typical of partial reinforcement training can reduce ESM in rats genetically selected for high levels of anxiety.