To meet the climate target set by the 2016 Paris Agreement and prevent climate catastrophes, upscaled carbon dioxide removal (CDR) is required by the end of this century. Of the several CDR strategies being explored, placement of macroalgae to the sea floor is one of consideration by the United States Department of Energy and other groups. This process utilizes the photosynthetic capabilities of macroalgae to capture carbon. Carbon-rich macroalgae can then be transported to the deep ocean, storing the carbon through burial for centuries. Laboratory experiments were conducted to determine degradation rates of giant kelp, Macrocystis pyrifera, as a potential species for implementation. We examined the decomposition of kelp into dissolved inorganic and organic carbon and particulate organic carbon, differentiating major kelp components: pneumatocyst, stipe, blade, whole segments, and macerated whole segments. Incubations were conducted at 4°C and 14°C to investigate the temperature effects on fresh and injured frond degradation over a period of two weeks. Contrasting experimental designs based on polynylon PET bags versus glass bottle housings were also compared for their feasibility of tracking degraded carbon. Bag incubations showed evidence for gas permeability, and direct effects of temperature on degradation cannot be quantified. Results for the bag incubations at the two temperatures are documented here but should be considered independently due to the uncontrolled differences in experimental design. In each temperature regime, segments of whole fronds were compared to the degradation of individual kelp components. Results showed incubations at 14°C had greater degradation with decay rate constants from 0.017 to 0.102 day-1, greater dissolved organic carbon (DOC) release rate from 439 to 839 μmol C g −1 (WW) h−1, and 9 to 12% of carbon becoming total inorganic carbon (TIC). Incubations at 4°C have decay rate constants of 0.003 to 0.016 day-1, approximate DOC release rates from 211 to 758 μmol C g −1 (WW) h−1, and 0 to 16% of carbon becoming TIC. In both incubations, particulate organic carbon (0.3 μm- 2.6 μm) was 2 to 3% of the total carbon.
Bottle experiments compared segments of whole fronds (WS) with macerated whole segments (MAC). Temperature had no apparent effect on WS respiration rates and minimally decreased MAC respiration rates in warmer conditions. Warmer temperatures had a positive effect on dissolved inorganic carbon (DIC) accumulation for both WS and MAC. After two weeks, incubations at 14°C had decay rate constants of 0.008 and 0.02 day-1, DOC rates of 936 and 777 μmol C g −1 (WW) h−1, and 8% and 4% total carbon becoming DIC for WS and MAC conditions respectively. Average decay constants for MAC kelp at 4°C across the two-week period was the highest of all treatments which was not predicted. After two-weeks, incubations at 4°C had decay constants of 0.014 and 0.016 day-1 and DOC rates of 1,139 and 1,014 μmol C g −1 (WW) h−1 for WS and MAC respectively. No carbon was accounted for in the DIC fraction for either kelp treatment at 4°C.
Glass bottles are the preferred experimental vessel over bags for their control of gas exchange. Within the bag experiments, blades had the most similar degradation patterns as that of whole segments and are considered a main determinant in whole frond decomposition. Across the experimental designs, warmer conditions appear to have a positive effect on TIC accumulation for M. pyrifera despite the state of oxygen exposure and is more influential in TIC accumulation compared to the extent of kelp injury that was implemented here. To improve the benefits of kelp placement as an OCDR strategy, these results demonstrate intact kelp in colder waters have slower average degradation over the first two weeks of placement and lower degradation rates at the two week time point compared to macerated kelp which can improve the chances of carbon sequestration into ocean basins. However, colder waters increased the rate of DOC release more than 20% for both WS and MAC kelp in hypoxic environments which should be considered in its effects of the deep ocean carbon cycle.