Recent research indicates that late prehistoric Numic expansion onto the western slope of the southern Sierra Nevada was facilitated by a competitive edge held by migrating groups, mainly Great Basin­derived settlement and subsistence behaviors ne­tuned to the uncertainties of living in Little Ice Age mountain environments. But ethnographic, linguistic, and archaeological evidence also suggests widespread borrowing from California cultures and the development of complementary social and economic relationships with California groups. This indicates a negotiated and elastic, rather than an overtly competitive, process of territorial expansion.

Walnut Creek: Left Coast Press, 2012, 281 pp., $79 (cloth)

At 3,609 m. (11,840 ft.) elevation in the White Mountains of Eastern California is a site containing 216 rock features consisting of cairns, pits, and other stacked-rock constructions but very few artifacts. Two obsidian bifaces, two milling tools, and lichenometric dating point towards site occupation between 440 and 190 cal B.P., contemporaneous with the White Mountains Village Pattern, which was marked by intensive seasonal occupations of multi-family groups in the alpine ecozone of the range. Though the site’s features are similar to facilities associated with artiodactyl hunting across the American West, their diversity, abundance, and distribution are more consistent with ceremonially-oriented sites on the Plains, in the Mojave Desert, and especially on the Plateau. This, in conjunction with the site’s setting, suggests that there were ritual functions associated with the site, and that the ceremonial use of high-altitudes has been overlooked in the region’s research history.

BACKGROUND: Women are at increased risk for psychosocial stress-related anxiety disorders, yet mechanisms regulating this risk are unknown. Psychosocial stressors activate microglia, and the resulting neuroimmune responses that females exhibit heightened sensitivity to may serve as an etiological factor in their elevated risk. However, studies examining the role of microglia during stress in females are lacking. METHODS: Microglia were manipulated in the stress-sensitive locus coeruleus (LC) of female rats in the context of social stress in two ways. First, intra-LC lipopolysaccharide (LPS; 0 or 3 μg/side, n = 5-6/group), a potent TLR4 agonist and microglial activator, was administered. One hour later, rats were exposed to control or an aggressive social defeat encounter between two males (WS, 15-min). In a separate study, females were treated with intra-LC or intra-central amygdala mannosylated liposomes containing clodronate (m-CLD; 0 or 25 μg/side, n = 13-14/group), a compound toxic to microglia. WS-evoked burying, cardiovascular responses, and sucrose preference were measured. Brain and plasma cytokines were quantified, and cardiovascular telemetry assessed autonomic balance. RESULTS: Intra-LC LPS augmented the WS-induced burying response and increased plasma corticosterone and interleukin-1β (IL-1β). Further, the efficacy and selectivity of microinjected m-CLD was fully characterized. In the context of WS, intra-LC m-CLD attenuated the hypervigilant burying response during WS as well as the accumulation of intra-LC IL-1β. Intra-central amygdala m-CLD had no effect on WS-evoked behavior. CONCLUSIONS: These studies highlight an innovative method for depleting microglia in a brain region specific manner and indicate that microglia in the LC differentially regulate hypervigilant WS-evoked behavioral and autonomic responses.

While the coronavirus disease 2019 (COVID-19) pandemic placed a heavy burden on healthcare systems worldwide, it also induced urgent mobilisation of research teams to develop treatments preventing or curing the disease and its consequences. It has, therefore, challenged critical care research to rapidly focus on specific fields while forcing critical care physicians to make difficult ethical decisions. This narrative review aims to summarise critical care research -from organisation to research fields- in this pandemic setting and to highlight opportunities to improve research efficiency in the future, based on what is learned from COVID-19. This pressure on research revealed, i.e., (i) the need to harmonise regulatory processes between countries, allowing simplified organisation of international research networks to improve their efficiency in answering large-scale questions; (ii) the importance of developing translational research from which therapeutic innovations can emerge; (iii) the need for improved triage and predictive scores to rationalise admission to the intensive care unit. In this context, key areas for future critical care research and better pandemic preparedness are artificial intelligence applied to healthcare, characterisation of long-term symptoms, and ethical considerations. Such collaborative research efforts should involve groups from both high and low-to-middle income countries to propose worldwide solutions. As a conclusion, stress tests on healthcare organisations should be viewed as opportunities to design new research frameworks and strategies. Worldwide availability of research networks ready to operate is essential to be prepared for next pandemics. Importantly, researchers and physicians should prioritise realistic and ethical goals for both clinical care and research.