Bioclogging in rivers can detrimentally impact aquifer recharge. This is particularly so in dry regions, where losing rivers are common, and where disconnection between surface water and groundwater (leading to the development of an unsaturated zone) can occur. Reduction in riverbed permeability due to biomass growth is a time-variable parameter that is often neglected, yet permeability reduction from bioclogging can introduce order of magnitude changes in seepage fluxes from rivers over short (i.e., monthly) timescales. To address the combined effects of bioclogging and disconnection on infiltration, I developed numerical representations of bioclogging processes within 1D, 2D, and 3D variably-saturated flow models representing losing-connected and losing-disconnected rivers subject to Mediterranean climatic effects on riverbed sediments. I tested bioclogging formulations using a synthetic and field case study informed with biological data obtained from the Russian River, California, U.S.A. My findings show that modeled biomass growth reduced seepage for losing-connected and losing-disconnected rivers. However, for rivers undergoing disconnection, infiltration declines occurred only after the system was fully disconnected. Before full disconnection, biologically-induced permeability declines were not significant enough to offset the infiltration gains introduced by disconnection. The two effects combine to lead to a characteristic infiltration curve where peak infiltration magnitude and timing is controlled by permeability declines relative to hydraulic gradient gains. Biomass growth was found to hasten the onset of full disconnection; a condition I term ‘effective disconnection’. Biomass growth leading to sediment pore-space clogging and consumption of river Carbon and Nitrogen reveal fundamental controls on bioclogging and river nutrient cycling based on riverine sediment conditions. These results show that river infiltration can respond dynamically to bioclogging and subsequent permeability declines that are highly dependent on river connection status.