Previous work has found that the contingencies experienced at UK traffic lights can affect drivers behavior potentiallyleading to risky driving. However, these studies did not account for the sequences experienced at traffic lights. Thisexperiment seeks to rectify this. As with previous research we used an incidental go/no-go task in which colored shapeswere stochastically predictive of whether a response was required. The stimuli encoded the contingencies of traffic lightsand their appropriate response, for example, stimuli G was a go cue, mimicking the response to a green light. Crucially,cues were displayed in the sequences experienced at traffic lights. Supporting earlier work, the 50/50 cue that mimickedamber traffic lights was experienced as a go cue, and the stop cue that represented red lights was responded to as a neutralcue. The sequences seemed to enhance this pattern of learning with much larger effect sizes than previously found.

The reaction time (RT) version of the Perruchet Effect is basedon a concurrent dissociation between RTs to respond andconscious expectancy of the outcome across runs of repeatedtrials. Consequently, the Perruchet Effect is considered strongevidence for multiple learning processes. This conclusion,however, relies on the RT trend being driven by associativelearning rather than, as some have argued, US recency orpriming mechanisms. Recent research examining themechanisms underlying the RT trend do so by examiningmotor activity associated with the response. With this aim inmind, the current study developed, and assessed the usefulnessof, a novel method to measure changes in the amount of forceapplied to the response button in an RT Perruchet paradigm.The results obtained could not be explained by a singlemechanism, but suggest multiple factors underlying the RTversion of the Perruchet effect.

To improve road safety, it is important to understand the impact that the contingencies around traffic lights have upon drivers’ behavior. There are formal rules that govern behavior at UK traffic lights (see The Highway Code, 2015), but what does experience of the contingencies do to us? While a green light always cues a go response and a singleton red a stop, the behavior linked to amber is ambiguous; in the presence of red it cues readiness to start, while on its own it cues "preparation" to stop. Could it be that the contingencies between stimuli and responses lead to implicit learning of responses that differ from those suggested by the rules of the road? This study used an incidental go/no-go task in which colored shapes were stochastically predictive of whether a response was required. The stimuli encoded the contingencies between traffic lights and their appropriate responses, for example, stimulus G was a go cue, mimicking the response to a green light. Evidence was found to indicate that G was a go cue, while A (which had the same contingencies as an amber light) was a weak go cue, and that R (a stop cue) was surprisingly responded to as a neutral cue.

Hard ticks (Acari: Ixodidae) have been historically identified by morphological methods which require highly specialized expertise and more recently by DNA-based molecular assays that involve high costs. Although both approaches provide complementary data for tick identification, each method has limitations which restrict their use on large-scale settings such as regional or national tick surveillance programs. To overcome those obstacles, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced as a cost-efficient method for the identification of various organisms, as it balances performance, speed, and high data output. Here we describe the use of this technology to validate the distinction of two closely related Dermacentor tick species based on the development of the first nationwide MALDI-TOF MS reference database described to date. The dataset obtained from this protein-based approach confirms that tick specimens collected from United States regions west of the Rocky Mountains and identified previously as Dermacentor variabilis are the recently described species, Dermacentor similis. Therefore, we propose that this integrative taxonomic tool can facilitate vector and vector-borne pathogen surveillance programs in the United States and elsewhere.

In 1953, investigators at the Rocky Mountain Laboratories in Hamilton, MT, described the isolation of a spotted fever group Rickettsia (SFGR) species from Dermacentor parumapertus ticks collected from black-tailed jackrabbits (Lepus californicus) in northern Nevada. Several decades later, investigators characterized this SFGR (designated the parumapertus agent) by using mouse serotyping methods and determined that it represented a distinct rickettsial serotype closely related to Rickettsia parkeri; nonetheless, the parumapertus agent was not further characterized or studied. To our knowledge, no isolates of the parumapertus agent remain in any rickettsial culture collection, which precludes contemporary phylogenetic placement of this enigmatic SFGR. To rediscover the parumapertus agent, adult-stage D. parumapertus ticks were collected from black-tailed jackrabbits shot or encountered as roadkills in Arizona, Utah, or Texas from 2011 to 2016. A total of 339 ticks were collected and evaluated for infection with Rickettsia species. Of 112 D. parumapertus ticks collected in south Texas, 16 (14.3%) contained partial ompA sequences with the closest identity (99.6%) to Rickettsia sp. strain Atlantic rainforest Aa46, an SFGR that is closely related or identical to an SFGR species that causes a mild rickettsiosis in several states of Brazil. A pure isolate, designated strain Black Gap, was cultivated in Vero E6 cells, and sequence analysis of the rrs, gltA, sca0, sca5, and sca4 genes also revealed the closest genetic identity to Rickettsia sp. Atlantic rainforest Aa46. Phylogenetic analysis of the five concatenated rickettsial genes place Rickettsia sp. strain Black Gap and Rickettsia sp. Atlantic rainforest Aa46 with R. parkeri in a distinct and well-supported clade.IMPORTANCE We suggest that Rickettsia sp. Black Gap and Rickettsia sp. Atlantic rainforest Aa46 represent nearly identical strains of R. parkeri and that Rickettsia sp. Black Gap or a very similar strain of R. parkeri represents the parumapertus agent. The close genetic relatedness among these taxa, as well as the response of guinea pigs infected with the Black Gap strain, suggests that R. parkeri Black Gap could cause disease in humans. The identification of this organism could also account, at least in part, for the remarkable differences in severity ascribed to Rocky Mountain spotted fever (RMSF) among various regions of the American West during the early 20th century. We suggest that the wide variation in case fatality rates attributed to RMSF could have occurred by the inadvertent inclusion of cases of milder disease caused by R. parkeri Black Gap.