Branched broomrape (Phelipanche ramosa), a parasitic weed that was the focus of a $1.5 million eradication effort four decades ago in California, has recently re-emerged in tomato fields in several Central Valley counties. Processing tomatoes are important to the California agricultural economy; the state produced over 90% of the 12 million tons of tomatoes grown in the United States in 2018. Branched broomrape is listed as an “A” noxious weed by the California Department of Food and Agriculture (CDFA); discovery of broomrape in California tomato fields leads to quarantine and crop destruction without harvest, resulting in significant economic loss to growers. In countries where broomrape is common, yield reductions caused by this parasitic weed can range from moderate to 80%, depending upon the infestation level, host and environmental conditions. Developing a detailed understanding of the biology of this weed under local conditions is an important step towards developing effective management plans for California. In this review, we discuss branched broomrape in the context of California production systems, particularly of tomato. We also discuss the potential management practices that could help to prevent or reduce the impacts of branched broomrape in tomatoes and other host crops.

Although dicamba has long been one of the most widely used selective herbicides, some U.S. states have banned the sale and use of dicamba because of farmers complaints of drift and damage to nonresistant crops. To prevent illegal use of dicamba and allow monitoring of nonresistant crops, a rapid and sensitive method for detection of dicamba is critical. In this paper, three novel dicamba haptens with an aldehyde group were synthesized, conjugated to the carrier protein via a reductive-amination procedure and an indirect competitive chemiluminescent enzyme immunoassay (CLEIA) for dicamba was developed. The assay showed an IC₅₀ of 0.874 ng/mL which was over 15 times lower than that of the conventional enzyme immunoassay. The immunoassay was used to quantify dicamba concentrations in field samples of soil and soybean obtained from fields sprayed with dicamba. The developed CLEIA showed an excellent correlation with LC-MS analysis in spike-and-recovery studies, as well as in real samples. The recovery of dicamba ranged from 86 to 108% in plant samples and from 105 to 107% in soil samples. Thus, this assay is a rapid and simple analytical tool for detecting and quantifying dicamba levels in environmental samples and potentially a great tool for on-site crop and field monitoring.