Studies of nematode assemblages in natural ecosystems can contribute to better understanding of the occurrence, relevance, and ecology of plant-parasitic and other soil nematodes. Nematode assemblages and environmental parameters (organic matter, water content (WC), bulk density (BD), total porosity (Po), soil respiration, and soil texture) were investigated in two seasons (rainy and dry) in two forest areas of the Zona da Mata, Pernambuco State. The aim of our research was to evaluate the heterogeneity between two locations and seasons in the Brazilian Atlantic Forest. Structure and composition of the nematode assemblages differed between areas and across time. Rhabditidae dominated the rainy season in both forest soils. Rarefaction curves (RC) suggest that sampling to detect more nematode taxa should be more intensive in the rainy season. The forest soils have complex, stable soil food webs with high connectance and decomposition channels dominated by bacteria. The predator-prey relationships were not affected by changes in soil properties that fluctuate with time.