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A software architecture for building power aware real time operating systems

ICS Technical Reports (2002)

As computing moves to battery operated portable systems, the functionality is increasingly implemented in software with an embedded/real-time operating system (RTOS). For such systems, there is a need for power-aware applications and system software. In this paper, we present a layered software architecture that enables the application and OS programmers to design energy-efficient applications and RTOS services. The software architecture consists of a power-aware RTOS kernel and a set of standard software interfaces that enable easy exchange of timing and power information among the underlying hardware platform, the RTOS, and the applications. To demonstrate the utility of our approach we focus on making the task scheduling process in an RTOS power-aware, and incorporate an OS-directed dynamic power management technique that enables adaptive power-fidelity tradeoffs during task scheduling. We have implemented it using the RedHat eCos operating system running on a complete variable voltage system based on the Intel XScale micro-architecture. We ran four different algorithms, from a simple shutdown based scheme to a dynamic predictive and adaptive DVS algorithm. The results show an energy gain of up to 66% when comparing to the execution without any power management incorporated and 17% comparing to the simple shutdown scheme.

Cover page: A software architecture for building power aware real time operating systems

Article
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Robust and artifact-free mounting of tissue samples for atomic force microscopy

UC Riverside Previously Published Works (2014)

Immobilization of tissue-samples for atomic for microscopy (AFM) is typically done using either semi-dry tissue or by gluing the tissue sample down, both of which can introduce artifacts. Here, we describe the design of a Soft- Clamping Immobilizing Retainer of Tissue (SCIRT) for consistent and nondestructive immobilization of tissues for AFM analysis. We compare the performance of our SCIRT method with glue-immobilization for two difficult to handle tissue types: human trabecular meshwork (HTM) and rabbit cornea (RC). Our results demonstrate that the SCIRT method has several advantages, including: (i) allowing for small sample sizes, (ii) enabling continuous hydration, (iii) eliminating contact with glue or associated solvents, (iv) permitting sample recovery following measurement, and (v) ease of use. In conclusion, the SCIRT method is a simple and effective means of immobilizing soft, hydrated tissue samples consistently and without artifacts.

Cover page: Robust and artifact-free mounting of tissue samples for atomic force microscopy

Article

Design Considerations for Solar Energy Harvesting Wireless Embedded Systems

Papers (2005)

Sustainable operation of battery powered wireless embedded systems (such as sensor nodes) is a key challenge, and considerable research effort has been devoted to energy optimization of such systems. Environmental energy harvesting, in particular solar based, has emerged as a viable technique to supplement battery supplies. However, designing an efficient solar harvesting system to realize the potential benefits of energy harvesting requires an in-depth understanding of several factors. For example, solar energy supply is highly time varying and may not always be sufficient to power the embedded system. Harvesting components, such as solar panels, and energy storage elements, such as batteries or ultracapacitors, have different voltage-current characteristics, which must be matched to each other as well as the energy requirements of the system to maximize harvesting efficiency. Further, battery nonidealities, such as self-discharge and round trip efficiency, directly affect energy usage and storage decisions. The ability of the system to modulate its power consumption by selectively deactivating its sub-components also impacts the overall power management architecture. This paper describes key issues and tradeoffs which arise in the design of solar energy harvesting, wireless embedded systems and presents the design, implementation, and performance evaluation of Heliomote, our prototype that addresses several of these issues. Experimental results demonstrate that Heliomote, which behaves as a plug-in to the Berkeley/Crossbow motes and autonomously manages energy harvesting and storage, enables near-perpetual, harvesting aware operation of the sensor node.

Cover page: Design Considerations for Solar Energy Harvesting Wireless Embedded Systems

Article
Peer Reviewed

Effect of Stratification on Surface Properties of Corneal Epithelial Cells.

UC Davis Previously Published Works (2015)

Purpose

The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.

Methods

hTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin.

Results

hTCEpi cells expressed the three main ocular mucins (MUC1, MUC4, and MUC16) with a maximum between days 1 and 3 of the stratification process. Upon stratification, cells caused a very significant increase in contact angle hysteresis, suggesting the development of spatially discrete and heterogeneously distributed surface features, defined by topography and/or chemical functionality. Although atomic force microscopy measurements showed no formation of appreciable topographic features on the surface of the cells, we observed a significant increase in surface chemical heterogeneity.

Conclusions

The surface chemical heterogeneity of the corneal epithelium may influence the dynamic behavior of tear film by "pinning" the contact line between the cellular surface and aqueous tear film. Engineering the surface properties of corneal epithelium could potentially lead to novel treatments in dry eye disease.

Cover page: Effect of Stratification on Surface Properties of Corneal Epithelial Cells.

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Species variation and spatial differences in mucin expression from corneal epithelial cells

UC Davis Previously Published Works (2016)

Mucins are large glycoproteins expressed by epithelial cells of both the conjunctiva and cornea, and principle components of the glycocalyx. They are thought to play an important role in determining the interactions between the cornea/conjunctiva and the overlying tear film. The purpose of this study was to characterize the membrane-associated corneal mucin expression pattern from multiple species commonly used in ophthalmic research and drug development to better define the biochemical attributes of the ocular surface. Humans, rhesus macaques and dogs were found to have a very similar pattern of mucin expression, with mucin 16 (MUC16) being the most prevalent mucin transcript. In contrast, the rabbit had a unique mucin expression pattern with all mucin transcripts expressed at relatively similar levels. To determine if there were spatial differences in expression, peripheral and central corneal epithelium were individually isolated and evaluated for mucin expression. In all species examined, MUC1, MUC4 and MUC16 had higher peripheral corneal expression when compared with central, which reached statistical significance in MUC1 (rhesus and dog). The data demonstrated variation in corneal epithelial membrane-associated mucin expression between species, with the rabbit having a distinct expression pattern. These differences may be reflective of the environment, pathogen exposure or tear film dynamics of the respective species. The species differences, as well as regional mucin expression patterns, characterized in this study further define the biochemical composition of the ocular surface and may play an important role in tear film stability.

Cover page: Species variation and spatial differences in mucin expression from corneal epithelial cells

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Squishy matters – Corneal mechanobiology in health and disease

UC Davis Previously Published Works (2024)

The cornea, as a dynamic and responsive tissue, constantly interacts with mechanical forces in order to maintain its structural integrity, barrier function, transparency and refractive power. Cells within the cornea sense and respond to various mechanical forces that fundamentally regulate their morphology and fate in development, homeostasis and pathophysiology. Corneal cells also dynamically regulate their extracellular matrix (ECM) with ensuing cell-ECM crosstalk as the matrix serves as a dynamic signaling reservoir providing biophysical and biochemical cues to corneal cells. Here we provide an overview of mechanotransduction signaling pathways then delve into the recent advances in corneal mechanobiology, focusing on the interplay between mechanical forces and responses of the corneal epithelial, stromal, and endothelial cells. We also identify species-specific differences in corneal biomechanics and mechanotransduction to facilitate identification of optimal animal models to study corneal wound healing, disease, and novel therapeutic interventions. Finally, we identify key knowledge gaps and therapeutic opportunities in corneal mechanobiology that are pressing for the research community to address especially pertinent within the domains of limbal stem cell deficiency, keratoconus and Fuchs' endothelial corneal dystrophy. By furthering our understanding corneal mechanobiology, we can contextualize discoveries regarding corneal diseases as well as innovative treatments for them.

Cover page: Squishy matters – Corneal mechanobiology in health and disease

Article
Peer Reviewed

Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells

UC Riverside Previously Published Works (2015)

Wnt antagonism has been linked to glaucoma and intraocular pressure regulation, as has increased stiffness of human trabecular meshwork (HTM) tissue. We have shown culturing HTM cells on substrates that mimic the elevated stiffness of glaucomatous tissue leads to elevated expression of the Wnt antagonist secreted frizzled related protein 1 (SFRP1), suggesting a linkage between SFRP1 and HTM mechanobiology. In this study, we document biomechanical consequences of Wnt antagonism on HTM cells. Cells were treated with the Wnt antagonists (SFRP1, KY02111, and LGK-974) for 8 days and allowed to recover for 4 days. After recovery, intrinsic cell stiffness and activation of the Wnt pathway via β-catenin staining and blotting were assayed. Basal cell stiffness values were 3.71 ± 0.37, 4.33 ± 3.07, and 3.07 ± kPa (median ± S.D.) for cells derived from 3 donors. Cell stiffness increased after 0.25 μg/mL (4.32 ± 5.12, 8.86 ± 8.51, 4.84 ± 3.15 kPa) and 0.5 μg/mL (16.75 ± 5.59, 13.18 ± 7.99, and 8.54 ± 5.77 kPa) SFRP1 treatment. Stiffening was observed after 10 μM KY02111 (10.72 ± 5.63 and 6.57 ± 5.53 kPa) as well as LGK-974 (9.60 ± 7.41 and 11.40 ± 9.24 kPa) treatment compared with controls (3.79 ± 1.01 and 5.16 ± 2.14 kPa). Additionally, Wnt inhibition resulted in decreased β-catenin staining and increased phosphorylation at threonine 41 after recovery. In conclusion, this work demonstrates a causal relationship between Wnt inhibition and cell stiffening. Additionally, these findings suggest transient Wnt inhibition resulted in durable modulation of the mechanical phenotype of HTM cells. When placed in context with previous results, these findings provide a causal link between Wnt antagonism and cell stiffness and suggest a feedback loop contributing to glaucoma progression.

Cover page: Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells

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