Inhalation exposure to pure and metabolic elevated carbon dioxide (CO₂) concentration has been associated with impaired work performance, lower perceived air quality, and increased health symptoms. In this study, the concentration of metabolic CO₂ was continuously measured in the inhalation zone of 41 subjects performing simulated office work. The measurements took place in an environmental chamber with well-controlled mechanical ventilation arranged as an office environment. The results showed the existence of a personal CO₂ cloud in the inhalation zone of all test subjects, characterized by the excess of metabolic CO₂ beyond the room background levels. For seated occupants, the median CO₂ inhalation zone concentration levels were between 200 and 500 ppm above the background, and the third quartile up to 800 ppm above the background. Each study subject had distinct magnitude of the personal CO₂ cloud owing to differences in metabolic CO₂ generation, posture, nose geometry, and breathing pattern. A small desktop oscillating fan proved to be suitable for dispersing much of the personal CO₂ cloud, thus reducing the inhalation zone concentration to background level. The results suggest that background measurements cannot capture the significant personal CO₂ cloud effect in human microclimate.