The introduction of novel perennial grains into annual row crop rotations is proposed to increase soil ecosystem services and enhance plant-soil-microbial linkages because perennials provide deeper root systems and more continuous ground cover than annuals. While soil microbial communities underpin many ecosystem services, we know little about how soil microbial composition and diversity, and soil carbon storage, differ between soils of annual vs. perennial grain crops. We measured soil fungi: bacteria (F/B) ratios and soil carbon within the novel perennial intermediate wheatgrass (IWG; trademarked Kernza®) and tilled annual wheat and compared soil microbial diversity and community composition within their rhizosphere, shallow bulk soil (0–15 cm) and total bulk soil (0–90 cm). After three years, soil depth explained 30–40% and 12–22% of the variance in bacterial and fungal community composition, respectively, while crop type explained 10% and 9–16% of the variance, respectively. Fungal communities were most impacted by crop type in the rhizosphere and shallow bulk soil and less sensitive to differences in soil depth. In contrast, crop type had a smaller effect on bacterial communities which were more influenced by soil depth. IWG trended higher in soil carbon mass at 0–30 cm (p = 0.22) and had a higher (F/B) ratio than tilled annual wheat at depths below 15 cm, but tilled annual wheat had higher soil carbon concentration (p = 0.12) and soil carbon mass (p = 0.09) at the 60–90 cm soil depth. Our results indicate that fungi were more responsive than bacterial communities to crop type and that IWG has a higher fungal biomass and different fungal community composition than annual wheat at depth. However, despite these distinct differences in fungal communities in IWG compared to annual wheat, the differences did not translate into greater soil carbon mass in IWG at depth.

Soil and soil biodiversity play critical roles in Nature's Contributions to People (NCP) # 10, defined as Nature's ability to regulate direct detrimental effects on humans, and on human-important plants and animals, through the control or regulation of particular organisms considered to be harmful. We provide an overview of pathogens in soil, focusing on human and crop pathogens, and discuss general strategies, and examples, of how soils' extraordinarily diverse microbial communities regulate soil-borne pathogens. We review the ecological principles underpinning the regulation of soil pathogens, as well as relationships between pathogen suppression and soil health. Mechanisms and specific examples are presented of how soil and soil biota are involved in regulating pathogens of humans and plants. We evaluate how specific agricultural management practices can either promote or interfere with soil's ability to regulate pathogens. Finally, we conclude with how integrating soil, plant, animal and human health through a 'One Health' framework could lead to more integrated, efficient and multifunctional strategies for regulating detrimental organisms and processes. This article is part of the theme issue 'The role of soils in delivering Nature's Contributions to People'.