One of the challenges of teaching is engaging students in a subject they may not see as relevant to them. This issue is especially prevalent when teaching statistics to health science students as many do not consider statistics an important piece of their medical training. Additional difficulty is presented when teaching courses via distance technology or courses that are partially or completely online as the valuable class discussion component is lost. This paper focuses on fostering “discussion” about statistical concepts and how they relate to each student on an individual level. This paper describes the online discussion board as a tool incorporated to supplement classroom activities and not as one to be limited to the online class. Two activities where the discussion board can be utilized are described: one where students participate in a series of guided discussions through instructor provided, thought-provoking questions and another where students critique an article related to their field of study and post for discussion. The objectives are to enhance knowledge, develop critical thinking, gain an appreciation of how statistics is used in different fields, and provide opportunities for discussion outside the classroom. Students are able to discuss issues with classmates who can be in the classroom or abroad using a virtual environment. This approach has been successfully used in both purely online classes and in large graduate level biostatistics classes including both synchronous and asynchronous distance learners.

The differences among individual eicosanoids in eliciting different physiological and pathological responses are largely unknown because of the lack of valid and simple analytical methods for the quantification of individual eicosanoids and their metabolites in serum, sputum and bronchial alveolar lavage fluid (BALF). Therefore, a simple and sensitive LC-MS/MS method for the simultaneous quantification of 34 eicosanoids in human serum, sputum and BALF was developed and validated. This method is valid and sensitive with a limit of quantification ranging from 0.2 to 3 ng/mL for the various analytes, and has a large dynamic range (500 ng/mL) and a short run time (25 min). The intra- and inter-day accuracy and precision values met the acceptance criteria according to US Food and Drug Administration guidelines. Using this method, detailed eicosanoid profiles were quantified in serum, sputum and BALF from a pilot human study. In summary, a reliable and simple LC-MS/MS method to quantify major eicosanoids and their metabolites was developed and applied to quantify eicosanoids in human various fluids, demonstrating its suitability to assess eicosanoid biomarkers in human clinical trials.