The non-coding 7SL RNA transcript complexes with six SRP proteins to form the Signal Recognition Particle. 7SL RNAs are produced with encoded U-tails of variable length due to their transcription by RNA polymerase III, and about 80% of endogenous 7SL RNAs receive one or two adenines at their 3’ ends by noncanonical poly(A) polymerases including TENT2. However, the relationship between SRP protein binding, 7SL RNA stability, and 3’ end tailing dynamics is not understood. To begin to investigate this, I established an exogenous 7SL1 RNA expression system with point mutations serving as barcodes for distinguishing from endogenous 7SL RNAs. Two independent exogenous barcoded 7SL1 RNAs, however, showed less 3' end adenylation and more uridylation than endogenous 7SL RNAs. A subsequent titration experiment revealed increased 3’ end adenylation and decreased uridylation at lower expression levels but still did not fully match endogenous 7SL RNAs even when expressed at below 10% of endogenous levels. TENT2 knockout caused almost complete loss of adenylation of the exogenous barcoded 7SL1 RNAs but no change in accumulation, suggesting that adenylation is not important for stability. Mutations impairing association of 7SL1 RNA with SRP54 showed no effects on accumulation or uridylation but moderate increases in adenylation, suggesting SRP54 binding does not impact 7SL RNA 3’ end processing or stability in a major way. Mutations known to impair association of 7SL1 RNA with SRP9, however, demonstrated increased uridylation and decreased adenylation and accumulation, suggesting SRP9 assembly impacts 7SL RNA 3' end tailing and stability.