Immobilization and accumulation of Fe in physiologically inactive forms somewhere in the leaf has been suggested to be associated to chlorosis in Fe-deficient leaves. The localization of elements at tissue level is essential to understand the plant processes involved in inorganic ion homeostasis. In this study we have used LT-SEM-EDX microanalysis to investigate the spatial distribution of Fe at tissue level in peach leaves under Fe-sufficient and Fe-deficient status. Chlorotic leaves from field peach trees showed a preferential localization of Fe in spongy parenchyma.