The European wild rabbit in Australia threatens the sustainability of agriculture and conservation of native flora and fauna. Improved means of reducing these impacts are sought including effort to develop virally vectored immunocontraception (VVIC). VVIC for the wild rabbit involves complex interactions between the rabbit, myxoma virus and insect vectors of the virus. Development of the method includes not only reproductive molecular biology and genetics and manipulation of virus genetics, but also many problems in reproductive biology, ecology and population dynamics of the rabbit in diverse environments. Furthermore, epidemiology of enzootic myxomatosis, and behavior and population dynamics of several vector species of mosquito and flea must be considered. Some of these ecological problems are described with a brief description of the approach to experimental analysis.

Biomass values are predicted from the radar at various frequencies and polarizations and are compared to the actual biomass values. Predicted biomass levels are most accurate at P-band. Multiple incidence angle data also reveal that the incidence angle of the radar illumination affects the retrieval of biomass from the radar data even at HV polarizations. One consequence is that topographic information is required for mapping the biomass in areas of moderate topography. Also the inversion curve for biomass retrieval varies with season and environmental conditions.

Airborne SAR data gathered by the NASA/JPL three-frequency, polarimetric, radar system in winter, spring, and summer over the Bonanza Creek Experimental Forest, near Fairbanks, AK, are compared to estimates of whole-tree aboveground dry biomass from 21 forest stands and two clear-cuts. While C-band radar backscatter shows little sensitivity to biomass, L and P-band radar backscatter increase by more than 6 dB when biomass increases from 5 to 200 tons/ha. Using second-order polynomial regressions, biomass values are predicted from the radar at L and P-band and compared to actual biomass values. At P-band HV-polarization, the error in predicted biomass is about 30 of the actual biomass. When HV, HH, and VV-polarization are used together in the regression, the error in predicted biomass is about 20. Errors obtained using L-band data are a few percents larger. These errors are caused by uncertainties in actual stand biomass estimates, significant inner-stand spatial variations in biomass, unusual conditions of forest stands following natural disturbances, along with interactions of the radar signals with a complex three-dimensional structure of the canopy. Multiple incidence angle data reveal that the incidence angle θiof the radar illumination is also a factor influencing the retrieval of biomass, even at HV-polarization, when θi > 50° or θi< 25°. Finally, the radar response of the forest-and thereby the regression curves for biomas retrieval-are dependent on the seasonal and environmental conditions. © 1994 IEEE

Cell shapes and connectivities evolve over time as the colony changes shape or embryos develop. Shapes of intercellular interfaces are closely coupled with the forces resulting from actomyosin interactions, membrane tension, or cell-cell adhesions. Although it is possible to computationally infer cell-cell forces from a mechanical model of collective cell behavior, doing so for temporally evolving forces in a manner robust to digitization difficulties is challenging. Here, we introduce a method for dynamic local intercellular tension estimation (DLITE) that infers such evolution in temporal force with less sensitivity to digitization ambiguities or errors. This method builds upon previous work on single time points (cellular force-inference toolkit). We validate our method using synthetic geometries. DLITE's inferred cell colony tension evolutions correlate better with ground truth for these synthetic geometries as compared to tension values inferred from methods that consider each time point in isolation. We introduce cell connectivity errors, angle estimate errors, connection mislocalization, and connection topological changes to synthetic data and show that DLITE has reduced sensitivity to these conditions. Finally, we apply DLITE to time series of human-induced pluripotent stem cell colonies with endogenously expressed GFP-tagged zonulae occludentes-1. We show that DLITE offers improved stability in the inference of cell-cell tensions and supports a correlation between the dynamics of cell-cell forces and colony rearrangement.

Recessive alleles have been shown to directly affect both human Mendelian disease phenotypes and complex traits. Pedigree studies also suggest that consanguinity results in increased childhood mortality and adverse health phenotypes, presumably through penetrance of recessive mutations. Here, we test whether the accumulation of homozygous, recessive alleles decreases reproductive success in a human population. We address this question among the Namibian Himba, an endogamous agro-pastoralist population, who until very recently practiced natural fertility. Using a sample of 681 individuals, we show that Himba exhibit elevated levels of inbreeding, calculated as the fraction of the genome in runs of homozygosity (FROH). Many individuals contain multiple long segments of ROH in their genomes, indicating that their parents had high kinship coefficients. However, we do not find evidence that this is explained by first-cousin consanguinity, despite a reported social preference for cross-cousin marriages. Rather, we show that elevated haplotype sharing in the Himba is due to a bottleneck, likely in the past 60 generations. We test whether increased recessive mutation load results in observed fitness consequences by assessing the effect of FROH on completed fertility in a cohort of postreproductive women (n = 69). We find that higher FROH is significantly associated with lower fertility. Our data suggest a multilocus genetic effect on fitness driven by the expression of deleterious recessive alleles, especially those in long ROH. However, these effects are not the result of consanguinity but rather elevated background identity by descent.

STUDY OBJECTIVES: It has long been suspected that sleep is important for regulating body temperature and metabolic-rate. Hibernation, a state of acute hypothermia and reduced metabolic-rate, offers a promising system for investigating those relationships.

The opiate antagonist naloxone was effective in reducing self-abusive behavior in two mentally retarded clients with an extensive history of such behavior. Three doses of naloxone (0.1, 0.2, 0.4 mg) were compared with a vehicle solution in a double-blind, crossover design. Naloxone greatly attenuated self-abusive episodes in one client and eliminated them entirely in the second client. In addition, use of self-restraining behavior by one client was reduced. The findings suggested that some clients with self-injurious behavior may have disturbances of the endogenous opiate system. Maintenance of self-abuse by tonically elevated pain threshold and/or by the putative addictive characteristics of such behavior was discussed.

Temporal dynamic studies of ERS-1 SAR measurements in relation to the changing environmental and phenologic state of the forest canopy and of the forest ground layers during the winter/spring and fall/winter transitions of 1993 and 1993 are conducted. During these transition, changes in radar backscatter observed by ERS-1 are correlated to freezing and thawing of the soil and vegetation in order to determine the start and end of the growing season for the forest.