We discuss ridge flank environments in the ocean crust as habitats for subseafloor microbial life. Oceanic ridge flanks, areas far from the magmatic and tectonic influence of seafloor spreading, comprise one of the largest and least explored microbial habitats on the planet. We describe the nature of selected ridge flank crustal environments, and present a framework for delineating a continuum of conditions and processes that are likely to be important for defining subseafloor microbial "provinces." The basis for this framework is three governing conditions that help to determine the nature of subseafloor biomes: crustal age, extent of fluid flow, and thermal state. We present a brief overview of subseafloor conditions, within the context of these three characteristics, for five field sites where microbial studies have been done, are underway, or have been proposed. Technical challenges remain and likely will limit progress in studies of microbial ridge flank ecosystems, which is why it is vital to select and design future studies so as to leverage as much general understanding as possible from work focused at a small number of sites. A characterization framework such that as presented in this paper, perhaps including alternative or additional physical or chemical characteristics, is essential for achieving the greatest benefit from multidisciplinary microbial investigations of oceanic ridge flanks.

In 2016, temperature recorders were recovered, temperatures were measured, and fluid samples were collected from Vent 1, a high temperature (338°C) hydrothermal discharge site on the southern Cleft Segment of the Juan de Fuca Ridge. Coupled with previous sampling efforts, this collection represents a 32-year record of discharge from a single chimney structure, the longest record to date. Remarkably, the fluid has remained brine-dominated for more than three decades. This brine formed during phase separation and segregation prior to initial observations in 1984. Although the chloride concentration of the discharging fluid has decreased with time, the fluid temperature has remained nearly constant for at least 3.3 years and probably for 15 or even 22 years. Compositions of the discharging fluids are consistent with inputs from a deep-sourced brine, which was last equilibrated at >400°C at a depth consistent with the base of the sheeted dikes and the brittle-ductile transition. This brine mixed (diffusion or dispersion) with a likely non-phase-separated, hydrothermal fluid prior to discharge. A survey of hydrothermal endmember fluids with chlorinities in excess of 700 mmol/kg shows, with the exception of Fe, a single trend between major ion concentrations and chlorinity even though data are from a range of crustal compositions, spreading rates, and water and magma depths. Calculated deep-sourced brines from hydrothermal fluid data are similar to data based on fluid inclusions and estimates of brine assimilation in magmas. A better understanding of brines is required given their potential duration of discharge and capacity for mobilizing metals.

Hydrothermal vent fields located in the gap between known sites in Guaymas Basin and 21°N on the East Pacific Rise were discovered on the Alarcón Rise and in southern Pescadero Basin. The Alarcón Rise spreading segment was mapped at 1-m resolution by an autonomous underwater vehicle. Individual chimneys were identified using the bathymetric data. Vent fields were interpreted as active from temperature anomalies in water column data and observed and sampled during remotely operated vehicle dives. The Ja Sít, Pericú, and Meyibó active fields are near the eruptive fissure of an extensive young lava flow. Vent fluids up to 360 °C from Meyibó have compositions similar to northern East Pacific Rise vents. The Tzab-ek field is 850 m west of the volcanic axis, and active chimneys rise up to 33 m above a broad sulfide mound. The inactive field is 10 km north-northeast along the rift axis, and most sulfide chimneys are enriched in Zn and associated elements that are transported at lower temperature compared to the more Cu-rich active fields. In southern Pescadero Basin, the Auka field is on the margin of a sediment-filled graben at 3,670-m depth. Discharging fluids are clear, contain hydrocarbons, and have neutral pH, elevated salinity, and temperatures up to 291 °C. They have deposited massive mounds of calcite with minor sulfide. The fluids are compositionally similar to those in Guaymas Basin, produced by high-temperature basalt-seawater interaction followed by reaction with sediment. The paucity of sulfide minerals suggests subsurface deposition of metals.