- Wood, Andrew J;
- Lo, Te-Wen;
- Zeitler, Bryan;
- Pickle, Catherine S;
- Ralston, Edward J;
- Lee, Andrew H;
- Amora, Rainier;
- Miller, Jeffrey C;
- Leung, Elo;
- Meng, Xiangdong;
- Zhang, Lei;
- Rebar, Edward J;
- Gregory, Philip D;
- Urnov, Fyodor D;
- Meyer, Barbara J
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.