Prosocial behavior is usually defined as a set of actions aimed to benefit others. This study aims at presenting validity and reliability evidence for the Brazilian version of the Prosocial Tendencies Measure (PTM). The study was conducted with 555 students (Mage= 23,38; SD = 6,24) from public and private universities in southeastern Brazil, 69% being female. The scale showed good evidence of content validity. The internal 6-factor structure was tested by Confirmatory Factor Analysis and showed acceptable goodness-of-fit indices. The Multigroup Confirmatory Factor Analysis (MCFCA) accepted configural, metric and scalar invariance, indicating that the PTM is an equivalent measure for men and women of different age groups. No unexpected response patterns to the instrument’s items were found. Evidence of convergent validity was observed with the Prosociality Measurement Scale (EMPA). The reliability indices obtained indicated adequate internal consistency of the instrument. The adapted scale shows evidence of reliability and validity, making it suitable for use in the Brazilian context.