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The glucocorticoid receptor ligand-binding domain confers drug-gated protein regulation in C. elegans

  • Author(s): Monsalve, Gabriela
  • Yamamoto, Keith
  • Ward, Jordan
  • et al.

Published Web Location

https://www.biorxiv.org/content/10.1101/445833v1
No data is associated with this publication.
Abstract

Abstract Controlling protein activity and localization is a key tool in modern biology. Mammalian steroid receptor ligand-binding domains (LBDs) fusions have been used in a range of organisms and cell-types to inactivate proteins of interest until the cognate steroid ligand is applied. Here, we demonstrate that the glucocorticoid receptor LBD confers ligand-gated control of a heterologous gene expression system (Q system) and the DAF-16 transcription factor in C. elegans . These experiments demonstrate provide a powerful tool for temporal control of protein activity, and will bolster existing tools used to modulate gene expression and protein activity in this animal.

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