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Open Access Publications from the University of California

Sequencing of genomes from environmental single cells

  • Author(s): Bowers, RM
  • Lee, J
  • Woyke, T
  • et al.

© Springer Science+Business Media, LLC 2018. Sequencing of single bacterial and archaeal cells is an important methodology that provides access to the genetic makeup of uncultivated microorganisms. We here describe the high-throughput fluorescence-activated cell sorting-based isolation of single cells from the environment, their lysis and strand displacement-mediated whole genome amplification. We further outline 16S rRNA gene sequence-based screening of single-cell amplification products, their preparation for Illumina sequencing libraries, and finally propose computational methods for read and contig level quality control of the resulting sequence data.

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