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Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming

  • Author(s): Matheu, MP
  • Othy, S
  • Greenberg, ML
  • Dong, TX
  • Schuijs, M
  • Deswarte, K
  • Hammad, H
  • Lambrecht, BN
  • Parker, I
  • Cahalan, MD
  • et al.

Published Web Location

https://doi.org/10.1038/ncomms7219
No data is associated with this publication.
Abstract

© 2015 Macmillan Publishers Limited. All rights reserved. Foxp3+regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate more rapidly than conventional T cells (Tconv), extend longer processes and interact with resident dendritic cells (DC) and Tconv. Tregs intercept immigrant DCs and interact with antigen-induced DC:Tconv clusters, while continuing to form contacts with activated Tconv. During antigen-specific responses, blocking CTLA4-B7 interactions reduces Treg-Tconv interaction times, increases the volume of DC:Tconv clusters and enhances subsequent Tconv proliferation in vivo. Our results demonstrate a role for altered cellular choreography of Tregs through CTLA4-based interactions to limit T-cell priming.

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This item is under embargo until December 31, 2999.