A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat.
- Qi, LL;
- Echalier, B;
- Chao, S;
- Lazo, GR;
- Butler, GE;
- Anderson, OD;
- Akhunov, ED;
- Dvorák, J;
- Linkiewicz, AM;
- Ratnasiri, A;
- Dubcovsky, J;
- Bermudez-Kandianis, CE;
- Greene, RA;
- Kantety, R;
- La Rota, CM;
- Munkvold, JD;
- Sorrells, SF;
- Sorrells, ME;
- Dilbirligi, M;
- Sidhu, D;
- Erayman, M;
- Randhawa, HS;
- Sandhu, D;
- Bondareva, SN;
- Gill, KS;
- Mahmoud, AA;
- Ma, X-F;
- Miftahudin;
- Gustafson, JP;
- Conley, EJ;
- Nduati, V;
- Gonzalez-Hernandez, JL;
- Anderson, JA;
- Peng, JH;
- Lapitan, NLV;
- Hossain, KG;
- Kalavacharla, V;
- Kianian, SF;
- Pathan, MS;
- Zhang, DS;
- Nguyen, HT;
- Choi, D-W;
- Fenton, RD;
- Close, TJ;
- McGuire, PE;
- Qualset, CO;
- Gill, BS
- et al.
Published Web Location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448828/Abstract
Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.
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