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A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat

  • Author(s): Qi, LL
  • Echalier, B
  • Chao, S
  • Lazo, GR
  • Butler, GE
  • Anderson, OD
  • Akhunov, ED
  • Dvořák, J
  • Linkiewicz, AM
  • Ratnasiri, A
  • Dubcovsky, J
  • Bermudez-Kandianis, CE
  • Greene, RA
  • Kantety, R
  • La Rota, CM
  • Munkvold, JD
  • Sorrells, SF
  • Sorrells, ME
  • Dilbirligi, M
  • Sidhu, D
  • Erayman, M
  • Randhawa, HS
  • Sandhu, D
  • Bondareva, SN
  • Gill, KS
  • Mahmoud, AA
  • Ma, XF
  • Miftahudin
  • Gustafson, JP
  • Conley, EJ
  • Nduati, V
  • Gonzalez-Hernandez, JL
  • Anderson, JA
  • Peng, JH
  • Lapitan, NLV
  • Hossain, KG
  • Kalavacharla, V
  • Kianian, SF
  • Pathan, MS
  • Zhang, DS
  • Nguyen, HT
  • Choi, DW
  • Fenton, RD
  • Close, TJ
  • McGuire, PE
  • Qualset, CO
  • Gill, BS
  • et al.

Published Web Location

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448828/
No data is associated with this publication.
Abstract

Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146). genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.

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