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Embryonic stem cell-based mapping of developmental transcriptional programs.

  • Author(s): Mazzoni, Esteban O
  • Mahony, Shaun
  • Iacovino, Michelina
  • Morrison, Carolyn A
  • Mountoufaris, George
  • Closser, Michael
  • Whyte, Warren A
  • Young, Richard A
  • Kyba, Michael
  • Gifford, David K
  • Wichterle, Hynek
  • et al.

The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

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