Sosa, Enrique; Kim, Rachel; Rojas, Ernesto J; Hosohama, Linzi; Hennebold, Jon D; Orwig, Kyle E; Clark, Amander T

doi:10.1016/j.scr.2017.03.011

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An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

2017

Published Web Location

https://doi.org/10.1016/j.scr.2017.03.011

Abstract

The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

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