Skip to main content
eScholarship
Open Access Publications from the University of California

UC San Diego

UC San Diego Previously Published Works bannerUC San Diego

Best practices for fluorescence microscopy of the cyanobacterial circadian clock.

  • Author(s): Cohen, Susan E;
  • Erb, Marcella L;
  • Pogliano, Joe;
  • Golden, Susan S
  • et al.

Published Web Location

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479491/
No data is associated with this publication.
Abstract

This chapter deals with methods of monitoring the subcellular localization of proteins in single cells in the circadian model system Synechococcus elongatus PCC 7942. While genetic, biochemical, and structural insights into the cyanobacterial circadian oscillator have flourished, difficulties in achieving informative subcellular imaging in cyanobacterial cells have delayed progress of the cell biology aspects of the clock. Here, we describe best practices for using fluorescent protein tags to monitor localization. Specifically, we address how to vet fusion proteins and overcome challenges in microscopic imaging of very small autofluorescent cells.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Item not freely available? Link broken?
Report a problem accessing this item