UCLA

We are working on the development of a methodology that can increase our ability to read longer DNA fragments accurately at scale from the Illumina next-generation sequencing platform. This method takes advantage of existing technologies in DNA library preparation techniques, in combination with a barcoding strategy that can uniquely identify DNA fragments of up to 40kb. Currently, the method is being tuned on small 2 to 3 kb pUC19 vectors, and existing plasmids such as these are not suited for our proposed methodology. The goal of my project is to create a custom cosmid containing yeast genomic DNA fragments of up to ~30 kb in length, which are barcoded for deconvoluting distinct large DNA sequences. These custom cosmids will be used to validate the sequencing method for larger DNA sequences.