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Efficient mouse genome engineering by CRISPR-EZ technology
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https://doi.org/10.1038/nprot.2018.012Abstract
CRISPR-EZ achieves 100% delivery of Cas9/sgRNA RNPs by zygote electroporation, enabling efficient incorporation of indels, exon deletions, point mutations, and small insertions into the mouse genome, and outperforming microinjection-based methods.
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